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首页> 外文期刊>British Journal of Cancer >Vindesine receptors in cells of a human leukaemia cell line
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Vindesine receptors in cells of a human leukaemia cell line

机译：人白血病细胞系细胞中的长春地碱受体

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To determine whether vindesine receptors are present in human leukaemic cells, K562 cells (established from chronic myelogenous leukaemia in blastic crisis) were incubated with 3H-vindesine. Binding of 3H-vindesine increased with incubation time and with increase in number of K562 cells. However, when excessive amounts of nonradioactive vindesine were added, the 3H-vindesine was displaced. Binding of 3H-vindesine was only inhibited by vinblastine, vincristine and vindesine. These results suggest that K562 cells have receptors for vindesine and that these receptors are common to vinca alkaloids. Scatchard analysis showed that the number of vindesine receptors differed according to the kind of cells tested. K562 and a T-cell leukaemia-derived cell line, MOLT-4, had more receptors than an acute promyelocytic leukaemia-derived cell line, HL-60, and normal blood lymphocytes. The degree of vindesine affinity to receptors did not differ markedly among the above-mentioned cells.
机译：为了确定人白血病细胞中是否存在长春地辛受体，将K562细胞（由处于塑性危机中的慢性粒细胞白血病建立）与3H-长春地辛一起孵育。 3H-长春地碱的结合随着孵育时间和K562细胞数目的增加而增加。然而，当添加过量的非放射性长春地辛时，3H-长春地辛被置换了。 3H-长春地碱的结合仅被长春碱，长春新碱和长春地辛抑制。这些结果表明K562细胞具有长春地辛的受体，并且这些受体是长春花生物碱所共有的。斯卡查德分析表明，长春新碱受体的数量根据所测试细胞的种类而有所不同。 K562和T细胞白血病衍生细胞系MOLT-4比急性早幼粒细胞白血病衍生细胞HL-60和正常血淋巴细胞具有更多的受体。长春地碱对受体的亲和程度在上述细胞之间没有显着差异。

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