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首页> 外文期刊>British Journal of Cancer >Absence of morphological, chromosomal and antigenic changes in the K-562 cell line growing as localized or disseminated tumours in nude mice
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Absence of morphological, chromosomal and antigenic changes in the K-562 cell line growing as localized or disseminated tumours in nude mice

机译：在裸鼠中生长为局部或弥散性肿瘤的K-562细胞系中无形态，染色体和抗原变化

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Transplantation of K-562 cells into adult and newborn nude mice led to the development of localized s.c. and disseminated myelosarcomas, respectively. This age-associated, changing pattern of in vivo proliferation of K-562 cells derived from a single aliquot was consistently repeated throughout sequential passages. The only variable in this experimental system was the age of the recipient mice. Not only did the mice have an identical genetic background, but also the transplanted K-562 cells were derived from a single culture passage. As shown by cytological and histological examinations, the characteristic morphology and percentage composition of the subpopulations of the K-562 cell line were preserved in successive in vitro and in vivo passages. The K-562 cells had no prevailing phenotypic traits which could be associated with the growth either in the s.c. tissue or in the viscera. Furthermore, the cells maintained the human karyotype, including their typical chromosomal abnormalities and antigenic determinants, as demonstrated by the binding of a specific antibody, throughout all passages. Our results demonstrate that heterotransplanted K-562 cells may change their behaviour in vivo without undergoing modifications associated with different types of growth. These findings would indicate that the ability of neoplastic cells to proliferate in various environments (metastases) is not the consequence of predetermined cellular characteristics but is functionally conditioned.
机译：将K-562细胞移植到成年和新生裸鼠中导致局部皮囊的发展。和散布的骨髓瘤。在整个连续传代过程中，始终如一地重复这种与年龄相关的，不断变化的源自单份等分试样的K-562细胞增殖模式。该实验系统中唯一的变量是受体小鼠的年龄。小鼠不仅具有相同的遗传背景，而且移植的K-562细胞均来自单个培养传代。如细胞学和组织学检查所示，在连续的体外和体内传代中，K-562细胞系的亚群的特征形态和百分比组成得以保留。 K-562细胞没有普遍的表型性状，这可能与皮下生长有关。组织或内脏。此外，这些细胞在整个传代过程中都保持了人类核型，包括其典型的染色体异常和抗原决定簇，这通过特异性抗体的结合得以证明。我们的结果表明，异种移植的K-562细胞可能会改变其体内行为，而不会经历与不同类型的生长相关的修饰。这些发现将表明，肿瘤细胞在各种环境（转移）中的增殖能力不是预定细胞特征的结果，而是功能性调节的。

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