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首页> 外文期刊>British Journal of Cancer >Sensitive detection of occult Ewing's cells by the reverse transcriptase-polymerase chain reaction
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Sensitive detection of occult Ewing's cells by the reverse transcriptase-polymerase chain reaction

机译：通过逆转录酶-聚合酶链反应灵敏检测隐匿性尤文细胞

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Recently, Ewing's tumours have been shown to carry specific hybrid transcripts resulting from the fusion of the EWS gene with FLI-1 or ERG genes. Based on the sensitivity and specificity of the detection of these alterations by the reverse transcriptase-polymerase chain reaction technique, we have developed an assay to search for small numbers of Ewing cells in various sites from patients with Ewing's tumour. This method enables the detection of fewer than one tumour cell per million blood mononuclear cells. A total of 28 primary sites and 51 peripheral samples from 36 patients were investigated. Tumour cells could be detected in 4/18 blood samples, 4/15 bone marrow aspirates and 2/18 peripheral stem cell harvests. EWS/FLI-1 and EWS/ERG transcripts being observed in eight and two cases respectively. The type of fusion transcript detected in peripheral site(s) was identical to that observed in the primary site. At diagnosis 5/16 patients (31%) demonstrated either circulating tumour cells or/and occult bone marrow metastasis. After induction therapy, tumour cells were detected in 3/21 patients. This highly sensitive method should be a relevant tool to allow a more accurate clinical assessment of the dissemination of Ewing's tumours.
机译：近来，尤因氏肿瘤已经显示出携带特异性杂种转录本，这是由于EWS基因与FLI-1或ERG基因融合所致。基于通过逆转录酶-聚合酶链反应技术检测这些改变的敏感性和特异性，我们开发了一种测定方法，以从尤因氏肿瘤患者的各个部位搜索少量的尤因细胞。该方法能够检测到百万分之一的血液单核细胞中少于一个的肿瘤细胞。共调查了来自36位患者的28个主要部位和51个外围样本。可以在4/18血液样本，4/15骨髓抽吸物中和2/18外周干细胞收获物中检测到肿瘤细胞。 EWS / FLI-1和EWS / ERG成绩单分别在八和两个案例中被观察到。在外周位点中检测到的融合转录物的类型与在初级位点中观察到的相同。在诊断时，有5/16名患者（31％）表现出循环肿瘤细胞或/和隐匿性骨髓转移。诱导治疗后，在3/21例患者中检测到肿瘤细胞。这种高度敏感的方法应该是一种相关工具，可以对尤因肿瘤的传播进行更准确的临床评估。

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