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首页> 外文期刊>British Journal of Cancer >The detection of melanoma cells in peripheral blood by reverse transcription-polymerase chain reaction
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The detection of melanoma cells in peripheral blood by reverse transcription-polymerase chain reaction

机译：逆转录-聚合酶链反应检测外周血中黑色素瘤细胞

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Both cutaneous and uveal melanoma undergo haematogenous dissemination. Detection of tyrosinase mRNA by reverse transcription-polymerase chain reaction (RT-PCR) has been described as an extremely sensitive way of detecting circulating viable melanoma cells in the peripheral venous blood, and this technique may be of value in the early detection of dissemination. Also, it has been suggested that surgical manipulation of the eye, such as occurs during enucleation, can provoke uveal melanoma dissemination. The purpose of this study was to evaluate whether tyrosinase mRNA is detectable in the peripheral blood of patients with uveal and cutaneous melanoma and in patients with uveal melanoma undergoing surgical procedures on the eye harbouring the tumour. Venous blood samples from 36 patients diagnosed as having active uveal melanoma and from six patients with advanced metastatic cutaneous melanoma were analysed. In addition, blood samples were spiked with known numbers of cells from three cell lines and four primary uveal melanoma cultures. The reported sensitivity of the technique was confirmed, with an ability to detect down to one cell per ml of blood. All 51 blood samples from the 36 patients with uveal melanoma were negative, and this included 20 perioperative blood samples. The test was also negative for the six patients with advanced cutaneous melanoma. There were two positives among 31 control samples analysed. This study demonstrates that there are far fewer circulating viable melanocytes than has been previously supposed in patients with melanoma and that the RT-PCR is of no clinical value in detecting metastatic melanoma disease. There was no evidence for surgery causing a bolus of melanoma cells to enter the peripheral circulation.
机译：皮肤和葡萄膜黑色素瘤均发生血源性播散。通过逆转录-聚合酶链反应（RT-PCR）检测酪氨酸酶mRNA被描述为检测外周静脉血液中循环的黑色素瘤细胞的一种极其灵敏的方法，该技术在早期检测传播中可能有价值。而且，已经提出，诸如在摘除过程中发生的眼的外科手术操作可引起葡萄膜黑色素瘤的扩散。这项研究的目的是评估是否在葡萄膜和皮肤黑色素瘤患者以及接受手术治疗的葡萄膜黑色素瘤患者的眼睛周围血液中检测到酪氨酸酶mRNA。分析了36名被诊断为活动性葡萄膜黑色素瘤的患者和6名晚期转移性皮肤黑色素瘤患者的静脉血样。此外，血样中掺入了来自三种细胞系和四种原发葡萄膜黑色素瘤培养物的已知数量的细胞。证实了该技术报道的敏感性，并且能够检测到每毫升血液中只有一个细胞的能力。 36例葡萄膜黑色素瘤患者的所有51份血样均为阴性，其中包括20例围手术期血样。六名晚期皮肤黑素瘤患者的检查结果均为阴性。分析的31个对照样品中有两个阳性。这项研究表明，黑色素瘤患者体内循环的黑色素细胞比以前所认为的要少得多，而且RT-PCR在检测转移性黑色素瘤疾病方面没有临床价值。没有证据表明手术会导致大量黑色素瘤细胞进入周围循环。

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