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首页> 外文期刊>Clinical Chemistry: Journal of the American Association for Clinical Chemists >Determination of beta 2-microglobulin in serum by a microparticle-enhanced nephelometric immunoassay.
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Determination of beta 2-microglobulin in serum by a microparticle-enhanced nephelometric immunoassay.

机译：微粒增强的浊度免疫测定法测定血清中的β2-微球蛋白。

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This fully automated nephelometric immunoassay to quantify beta 2-microglobulin in human serum measures the light-scattering signal produced by agglutination of commercially available latex microparticles (diameter 0.1 micron) coated with specific F(ab')2 against beta 2-microglobulin. The calibration curve, generated by serial dilutions of a beta 2-microglobulin standard of known concentration, is used to calculate beta 2-microglobulin concentrations in serum samples by the logit-log function and linear-regression analysis. The assay range (sample dilution 400-fold) extends from 0.3 to 40.0 mg/L. No antigen excess appears at beta 2-microglobulin concentrations up to 320 mg/L. Within-run CVs ranged from 1.0% to 3.4%, and between-days from 1.2% to 2.8%. Total imprecision (CV) was 5%. Analytical recovery averaged 99.5% +/- 2.8%. Rheumatoid factor, complement, bilirubin (up to 340 mumol/L), and hemoglobin (up to 2.0 g/L) do not interfere. Strongly turbid lipemic samples must be cleared before analysis. Standard curve linearity was very good in samples covering the clinical useful range of concentrations. Results of the method correlated well with those of radioimmunoassay and microparticle enzyme-linked immunoassay (r = 0.979 and 0.975, respectively). The reference interval (nonparametric estimation) in apparently healthy adults (n = 303) was 0.87 (0.80-0.94) to 2.42 (2.28-2.45) mg/L; the median value was 1.54 mg/L.
机译：这项用于定量测定人血清中β2-微球蛋白的全自动浊度免疫测定法可测量由凝集有针对β2-微球蛋白的特定F（ab'）2的市售乳胶微粒（直径0.1微米）凝集产生的光散射信号。通过连续稀释已知浓度的β2-微球蛋白标准液产生的校准曲线，通过对数-对数函数和线性回归分析，用于计算血清样品中的β2-微球蛋白浓度。测定范围（样品稀释400倍）从0.3到40.0 mg / L。在β2-微球蛋白浓度高达320 mg / L时，没有抗原过量出现。批量生产CV的范围从1.0％到3.4％，日间CV的范围从1.2％到2.8％。总不精确度（CV）小于5％。分析回收率平均为99.5％+/- 2.8％。类风湿因子，补体，胆红素（最高340摩尔/升）和血红蛋白（最高2.0克/升）不干扰。分析前必须清除混浊的脂类样品。在涵盖临床有用浓度范围的样品中，标准曲线线性非常好。该方法的结果与放射免疫分析和微粒酶联免疫分析的结果相关性很好（分别为r = 0.979和0.975）。显然健康的成年人（n = 303）的参考间隔（非参数估计）为0.87（0.80-0.94）至2.42（2.28-2.45）mg / L;中位数为1.54 mg / L。

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