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首页> 外文期刊>Clinical Chemistry: Journal of the American Association for Clinical Chemists >In vitro effect of elastase and cathepsin G from human neutrophils on creatine kinase and lactate dehydrogenase isoenzymes.
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In vitro effect of elastase and cathepsin G from human neutrophils on creatine kinase and lactate dehydrogenase isoenzymes.

机译：人类嗜中性粒细胞的弹性蛋白酶和组织蛋白酶G对肌酸激酶和乳酸脱氢酶同工酶的体外作用。

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摘要

The polymorphonuclear granulocyte, or neutrophil, has been implicated as a mediator of tissue-destructive events because it releases the preformed proteolytic enzymes elastase and cathepsin G, and, as a result of myeloperoxidase action, hypochlorous acid. We show that elastase inactivates and fragments creatine kinase isoenzymes CK-2 and CK-3, and, to a lesser extent, lactate dehydrogenase (LD) isoenzyme LD-1, whereas cathepsin G acts only on CK-2. Both neutrophil enzymes act on LD-3. The course of inactivation was followed by measuring the loss of catalytic activity at 37 degrees C. The evidence for fragmentation was obtained by gel filtration; electrophoresis after sample treatment with sodium dodecyl sulfate and 2-mercaptoethanol was less satisfactory for this purpose. Hypochlorous acid inactivates CK activity by about 75% at concentrations as low as 8 mumol/L and totally at concentrations 140 mumol/L, whereas LD activity is not affected until concentrations exceed 200 mumol/L. After a myocardial infarction, the number of neutrophils increases; they are triggered and concentrate around damaged myocardial tissue. Our data suggest that neutrophils may inactivate and fragment "cardiac" enzymes released from such damaged tissue.
机译：多形核粒细胞或嗜中性粒细胞被认为是组织破坏性事件的介质，因为它释放预先形成的蛋白水解酶弹性蛋白酶和组织蛋白酶G，并由于髓过氧化物酶的作用而释放次氯酸。我们显示，弹性蛋白酶灭活并断裂肌酸激酶同工酶CK-2和CK-3，以及在较小程度上乳酸脱氢酶（LD）同工酶LD-1，而组织蛋白酶G仅作用于CK-2。两种中性粒细胞酶均作用于LD-3。通过在37℃下测量催化活性的损失来追踪失活的过程。为此目的，用十二烷基硫酸钠和2-巯基乙醇处理样品后的电泳不太令人满意。次氯酸在低至8μmol/ L的浓度下和大约140μmol/ L的浓度下可使CK活性失活约75％，而LD活性直到浓度超过200μmol/ L才受影响。心肌梗塞后，中性粒细胞数量增加;它们被触发并集中在受损的心肌组织周围。我们的数据表明，嗜中性粒细胞可能使这种受损组织释放的“心脏”酶失活并破碎。

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