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首页> 外文期刊>Infection and immunity >In vitro killing of Actinobacillus actinomycetemcomitans and Capnocytophaga spp. by human neutrophil cathepsin G and elastase.
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In vitro killing of Actinobacillus actinomycetemcomitans and Capnocytophaga spp. by human neutrophil cathepsin G and elastase.

机译：放线放线放线杆菌和Capnocytophaga spp的体外杀伤。由人中性粒细胞组织蛋白酶G和弹性蛋白酶引起。

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摘要

The purpose of this study was to compare the killing of Actinobacillus actinomycetemcomitans with that of Capnocytophaga spp. by purified cathepsin G and elastase in vitro. Both were sensitive to killing by purified cathepsin G, but only the Capnocytophaga spp. were killed by elastase. Killing by cathepsin G exhibited logarithmic kinetics, was enhanced slightly by alkaline pH, and was enhanced greatly under hypotonic conditions. Treatment of cathepsin G with diisopropyl fluorophosphate significantly reduced its bactericidal activity against Capnocytophaga spp. but not against Escherichia coli or A. actinomycetemcomitans. The bactericidal effects of cathepsin G against Capnocytophaga sputigena and A. actinomycetemcomitans were inhibited by alpha-1-antichymotrypsin, alpha-1-antitrypsin, and alpha-2-macroglobulin but not by bovine serum albumin. We conclude that (i) cathepsin G kills Capnocytophaga spp. and A. actinomycetemcomitans, (ii) elastase kills Capnocytophaga spp., (iii) the bactericidal activity of cathepsin G is enzyme dependent against Capnocytophaga spp. and enzyme independent against A. actinomycetemcomitans, and (iv) natural plasma antiproteases may control both enzyme-dependent and enzyme-independent bactericidal activities of cathepsin G.
机译：这项研究的目的是比较放线菌放线杆菌和Capnocytophaga spp的杀伤力。纯化的组织蛋白酶G和弹性蛋白酶体外。两者均对纯化的组织蛋白酶G的杀伤敏感，但仅对Capnocytophaga spp杀伤敏感。被弹性蛋白酶杀死。组织蛋白酶G的杀伤表现出对数动力学，碱性pH的杀伤力略有增强，在低渗条件下的杀伤力大大增强。用磷酸二异丙酯处理组织蛋白酶G明显降低了其对Capnocytophaga spp的杀菌活性。但不针对大肠埃希氏菌或放线杆菌。组织蛋白酶G对痰液中的Capnocytophaga sputigena和A.放线菌的杀菌作用受到α-1-抗胰凝乳蛋白酶，α-1-抗胰蛋白酶和α-2-巨球蛋白的抑制，但不受牛血清白蛋白的抑制。我们得出的结论是（i）组织蛋白酶G杀死Capnocytophaga spp。 （ii）弹性蛋白酶杀死Capnocytophaga spp。，（iii）组织蛋白酶G的杀菌活性是依赖Capnocytophaga spp的酶。 （ii）天然血浆抗蛋白酶可以控制组织蛋白酶G的酶依赖性和非酶依赖性杀菌活性。

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