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首页> 外文期刊>Infection and immunity >Differential killing of Actinobacillus actinomycetemcomitans and Capnocytophaga spp. by human neutrophil granule components.
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Differential killing of Actinobacillus actinomycetemcomitans and Capnocytophaga spp. by human neutrophil granule components.

机译：差异杀伤放线放线杆菌和Capnocytophaga spp。由人中性粒细胞颗粒组成。

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The purpose of this study was to determine whether granule fractions of human neutrophils differentially kill Actinobacillus actinomycetemcomitans and Capnocytophaga spp. Granule extracts were subjected to gel filtration, and seven fractions (designated A through G) were obtained. Under aerobic conditions at pH 7.0, representative strains of A. actinomycetemcomitans were killed by fraction D and variably by fraction B. In contrast, the Capnocytophaga spp. were killed by fractions C, D, F, and G. Fractions A (containing lactoferrin and myeloperoxidase) and E (containing lysozyme) exerted little bactericidal activity under these conditions. Anaerobiosis had little effect on the bactericidal activity of fractions D and F but inhibited that of fractions B and C. Electrophoresis, zymography, determination of amino acid composition, and N-terminal sequence analysis revealed that fraction C contained elastase, proteinase 3, and azurocidin. Fraction D contained lysozyme, elastase, and cathepsin G. Subfractions of C and D containing elastase (subfraction C4), a mixture of elastase and azurocidin (subfraction C5), and cathepsin G (subfraction D9) were found to be bactericidal. The bactericidal effects of fraction D and subfraction D9 against A. actinomycetemcomitans was not inhibited by heat inactivation, phenylmethylsulfonyl fluoride, or N-benzyloxycarbonylglycylleucylphenylalanylchloromethyl ketone. We conclude that (i) A. actinomycetemcomitans and Capnocytophaga spp. were sensitive to the bactericidal effects of different neutrophil granule components, (ii) both were sensitive to the bactericidal effects of neutral serine proteases, and (iii) the killing of A. actinomycetemcomitans by cathepsin G-containing fractions was independent of oxygen and neutral serine protease activity.
机译：这项研究的目的是确定人类嗜中性粒细胞的颗粒级分是否能杀死放线放线杆菌和Capnocytophaga spp。将颗粒提取物进行凝胶过滤，得到7个级分（称为A至G）。在pH值为7.0的有氧条件下，代表性的放线放线杆菌菌株被D部分杀死，B部分被可变地杀死。相比之下，Capnocytophaga spp。在馏分C，D，F和G中将其杀死。馏分A（含有乳铁蛋白和髓过氧化物酶）和E（含有溶菌酶）在这些条件下几乎没有杀菌活性。厌氧菌对级分D和F的杀菌活性影响很小，但抑制级分B和C的杀菌活性。电泳，酶谱分析，氨基酸组成测定和N端序列分析表明，级分C含有弹性蛋白酶，蛋白酶3和天青霉素。 。馏分D含有溶菌酶，弹性蛋白酶和组织蛋白酶G。C和D的亚组分含有弹性酶（亚组分C4），弹性蛋白酶和天青霉素的混合物（亚组分C5）和组织蛋白酶G（亚组分D9）具有杀菌作用。通过热灭活，苯基甲基磺酰氟或N-苄氧基羰基糖基亮氨酰苯基丙氨酰氯甲基酮不抑制级分D和亚级分D9对放线曲霉的杀菌作用。我们得出的结论是（i）A.放线菌和Capnocytophaga spp。对不同嗜中性粒细胞颗粒成分的杀菌作用敏感;（ii）对中性丝氨酸蛋白酶的杀菌作用均敏感;（iii）含组织蛋白酶G的组分对放线杆菌的杀伤作用与氧气和中性丝氨酸无关蛋白酶活性。

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