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首页> 外文期刊>Clinical Chemistry: Journal of the American Association for Clinical Chemists >Identification of Ghrelin in Human Saliva: Production by the Salivary Glands and Potential Role in Proliferation of Oral Keratinocytes
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Identification of Ghrelin in Human Saliva: Production by the Salivary Glands and Potential Role in Proliferation of Oral Keratinocytes

机译：鉴定人类唾液中的Ghrelin：唾液腺的产生及其在口腔角质形成细胞增殖中的潜在作用

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Background: We investigated whether ghrelin is present in human saliva, is produced by salivary glands, and physiologic consequences of these findings.Methods: Expression of ghrelin and specific receptor mRNA was determined by PCR. Proteins were identified by immunoblotting and size-exclusion fast protein liquid chromatography (FPLC) with consecutive RIA. Specific RIAs were used for quantification of salivary total and bioactive ghrelin. Distribution of ghrelin was investigated by immunohistochemistry in cryosections of the salivary glands. The effect of ghrelin on incorporation of 5-bromo-2′-deoxyuridine as a measure of cell proliferation was investigated in primary oral keratinocytes.Results: Ghrelin is produced by the salivary glands. The hormone was identified in saliva and glands by immunoblotting and by FPLC fractionation of saliva. Immunohistochemistry demonstrated ghrelin distribution in the salivary glands. The receptor was also produced by the glands and by oral keratinocytes and was shown to be functional. Comparison of total ghrelin values for healthy individuals (body mass index, 18–27 kg/m2) showed significantly lower concentrations in saliva than in serum ( P 0.01). The correlation between both matrices was r 2 = 0.56 ( P 0.001) with a negative correlation to body mass index ( r 2 = 0.314; P 0.01). Bioactive acylated ghrelin was also present in saliva. Incubation of keratinocytes with ghrelin led to significantly increased cell proliferation ( P 0.001). This effect could be completely suppressed by co-incubation with NOX-B11 (50 nmol/L), a novel specific inhibitor of acylated ghrelin.Conclusions: Ghrelin in saliva is produced and released by salivary glands. The effect of ghrelin on oral cell proliferation adds to the pro-proliferative action of other salivary growth factors.
机译：背景：我们调查了人唾液中是否存在生长激素释放肽，是否由唾液腺产生，以及这些发现的生理后果。方法：通过PCR检测生长激素释放肽和特定受体mRNA的表达。通过免疫印迹法和尺寸排阻快速蛋白质液相色谱法（FPLC）进行连续RIA鉴定蛋白质。特定的RIA用于定量唾液中总和生物活性的生长素释放肽。通过免疫组织化学在唾液腺冷冻切片中研究了生长素释放肽的分布。研究了生长素释放肽对口服原代角质形成细胞中5-溴-2'-脱氧尿苷掺入的影响，以此作为细胞增殖的量度。结果：生长素释放肽由唾液腺产生。通过免疫印迹和唾液的FPLC分级鉴定了唾液和腺体中的激素。免疫组织化学表明，ghrelin在唾液腺中分布。该受体也由腺体和口腔角质形成细胞产生，并被证明具有功能。比较健康个体的总生长素释放肽值（体重指数，18-27 kg / m2），显示唾液中的浓度远低于血清中的浓度（P <0.01）。两种矩阵之间的相关性为r 2 = 0.56（P <0.001），与体重指数呈负相关（r 2 = 0.314; P <0.01）。唾液中也存在具有生物活性的酰化生长素释放肽。与生长素释放肽一起孵育角质形成细胞导致细胞增殖显着增加（P <0.001）。与NOX-B11（50 nmol / L）共同孵育可完全抑制这种作用，NOX-B11是一种新型的酰化生长激素释放肽的特异性抑制剂。结论：唾液中的生长激素释放肽由唾液腺产生并释放。生长激素释放肽对口腔细胞增殖的作用增加了其他唾液生长因子的促增殖作用。

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