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首页> 外文期刊>Applied and Environmental Microbiology >Syntrophomonas wolfei gen. nov. sp. nov., an Anaerobic, Syntrophic, Fatty Acid-Oxidizing Bacterium
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Syntrophomonas wolfei gen. nov. sp. nov., an Anaerobic, Syntrophic, Fatty Acid-Oxidizing Bacterium

机译：狼对虾十一月sp。 nov。，一种厌氧，营养，脂肪酸氧化细菌

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摘要

An anaerobic, nonphototrophic bacterium that β-oxidizes saturated fatty acids (butyrate through octanoate) to acetate or acetate and propionate using protons as the electron acceptor (H2 as electron sink product) was isolated in coculture with either a non-fatty acid-degrading, H2-utilizing Desulfovibrio sp. or methanogens. Three strains of the bacterium were characterized and are described as a new genus and species, Syntrophomonas wolfei. S. wolfei is a gram-negative, slightly helical rod with round ends that possesses between two to eight flagella laterally inserted along the concave side of the cell. It has a multilayered cell wall of the gram-negative type. The presence of muramic acid, inhibition of growth by penicillin, and increased sensitivity of the cells to lysis after treatment with lysozyme indicate that peptidoglycan is present in the cell wall. Cells of S. wolfei contain poly-β-hydroxybutyrate. Isoheptanoate was degraded to acetate, isovalerate, and H2. Carbohydrates, proteinaceous materials, alcohols, or other tested organic compounds do not support growth. Common electron acceptors are not utilized with butyrate as the electron donor. Growth and degradation of fatty acids occur only in syntrophic association with H2-using bacteria. The most rapid generation time obtained by cocultures of S. wolfei with Desulfovibrio and Methanospirillum hungatei is 54 and 84 h, respectively. The addition of Casamino Acids but neither Trypticase nor yeast extract stimulated growth and resulted in a slight decrease in the generation time of S. wolfei cocultured with M. hungatei. The addition of H2 to the medium stopped growth and butyrate degradation by S. wolfei.
机译：将质子作为电子受体（H2作为电子吸收产物）将厌氧，β-氧化饱和脂肪酸（丁酸通过辛酸）氧化为乙酸盐或乙酸盐和丙酸盐的厌氧，非营养细菌与非脂肪酸降解， H2利用Desulfovibrio sp。或产甲烷菌。表征了该细菌的三种菌株，并被描述为一种新的属和种，即Syntrophomonas wolfei。沃尔夫链球菌是一种革兰氏阴性的略带螺旋的杆，具有圆形末端，沿细胞凹面侧向插入有2至8个鞭毛。它具有革兰氏阴性类型的多层细胞壁。在用溶菌酶处理后，山梨酸的存在，青霉素对生长的抑制以及细胞对裂解的敏感性增加，表明肽聚糖存在于细胞壁中。德意志链球菌的细胞含有聚-β-羟基丁酸酯。异庚酸酯被降解为乙酸盐，异戊酸酯和H 2。碳水化合物，蛋白质材料，酒精或其他经过测试的有机化合物不支持生长。普通的电子受体不与丁酸酯一起用作电子供体。脂肪酸的生长和降解仅与使用H2的细菌发生营养性结合。沃尔夫链霉菌与脱硫弧菌和饥饿的甲基甲烷螺旋菌共培养获得的最快速的生成时间分别为54 h和84 h。添加酪蛋白氨基酸而不是胰蛋白酶和酵母提取物都不会刺激生长，并导致与饥饿树分枝杆菌共培养的狼尾草的生成时间略有减少。向培养基中添加H2可阻止狼毒链球菌的生长和丁酸盐降解。

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