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首页> 外文期刊>FEMS Microbiology Letters >Syntrophaceticus schinkiigen. nov., sp. nov., an anaerobic, syntrophic acetate-oxidizing bacterium isolated from a mesophilic anaerobic filter
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Syntrophaceticus schinkiigen. nov., sp. nov., an anaerobic, syntrophic acetate-oxidizing bacterium isolated from a mesophilic anaerobic filter

机译：食腐菌（Syntrophaceticus schinkiigen）。十一月，sp。 nov。，一种从嗜温厌氧滤池中分离出来的厌氧，可氧化的乙酸氧化细菌

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摘要

A mesophilic, syntrophic acetate-oxidizing bacterium, designated strain Sp3~T, was isolated from sludge from a mesophilic methanogenic digestor operating at a high ammonium concentration (6.4 g L~(-1) NH_4~+-N). The strain showed acetate-oxidizing ability in cocultivation with a hydrogen-consuming methanogen. Comparative 16S rRNA gene sequence analysis confirmed that strain Sp3~T belonged to the Firmicutes-Clostridia class. The most closely related species was Thermacetogenium phaeum (16S rRNA gene sequence identity 92%). Strain Sp3 ~T used ethanol, betaine and lactate as carbon and electron sources and showed growth between 25 and 40 °C and pH 6.0 and 8.0. Based on the phylogenetic position and the physiological characteristics of strain Sp3 ~T, this new syntrophic, acetate-oxidizing bacterium is proposed as the new genus and species Syntrophaceticus schinkii, with Sp3~T (=JCM 16669~T) as the type strain. An isolate (strain Esp=JCM 16670) with high 16S rRNA gene sequence identity (99%) to syntrophic acetate-oxidizing Clostridium ultunense was also retrieved from the methanogenic digestor.
机译：从以高铵浓度（6.4 g L〜（-1）NH_4〜+ -N）运行的中温产甲烷消化器的污泥中分离出了嗜温，营养性乙酸盐氧化菌，命名为Sp3〜T菌株。该菌株与耗氢的产甲烷菌共培养显示出乙酸盐氧化能力。比较的16S rRNA基因序列分析证实菌株Sp3〜T属于Firmicutes-Clostridia类。亲缘关系最密切的物种是Thermacetogenium phaeum（16S rRNA基因序列同一性为92％）。 Sp3〜T菌株使用乙醇，甜菜碱和乳酸作为碳源和电子源，并在25至40°C和pH 6.0至8.0之间生长。根据菌株Sp3〜T的系统发生位置和生理特性，提出了一种新的，能氧化的，能氧化的，能氧化的细菌，它是新的属和物种Shintrophaceticus schinkii，以Sp3〜T（= JCM 16669〜T）为菌株。还从产甲烷消化器中回收了与腐殖酸-乙酸氧化的梭状芽胞杆菌具有高16S rRNA基因序列同一性（99％）的分离株（菌株Esp = JCM 16670）。

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