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首页> 外文期刊>Applied and Environmental Microbiology >New selective agent for isolation of Pseudomonas aeruginosa.
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New selective agent for isolation of Pseudomonas aeruginosa.

机译：用于分离铜绿假单胞菌的新型选择剂。

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Results of minimal inhibitory concentration tests with a diversity of bacterial strains showed that 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan (C-390) inhibited the growth of all microorganisms tested (other than Pseudomonas aeruginosa) at 25 microgram/ml or less, whereas MICs obtained for P. aeruginosa ranged from to to greater than 100 microgram/ml. Therefore, C-390 was evaluated as a potential selective agent for P. aeruginosa in pseudomonas agar F. Recovery tests were conducted on this medium with 53 strains o P. aeruginosa, and the results were compared to those obtained in similar tests on commercially available selective media, i.e., pseudomonas isolation agar and Pseudosel agar. The results of these comparisons indicated that pseudomonas agar F with C-390 was significantly less inhibitory than Pseudosel agar and pseudomonas isolation agar and more selective than pseudomonas isolation agar. The incorporation of C-390 in pseudomonas agar F also provided a medium that was both selective and differential. Preliminary evidence also suggested that C-390 may be added to other basal media with comparable results.
机译：对多种细菌菌株进行最低抑菌浓度测试的结果表明，在25微克的浓度下，9-氯-9-（4-二乙基氨基苯基）-10-苯基ac啶酮（C-390）抑制了所有测试微生物（铜绿假单胞菌除外）的生长/ ml或更低，而获得的铜绿假单胞菌的MIC范围从到到大于100微克/ ml。因此，C-390被评估为假单胞菌琼脂F中铜绿假单胞菌的潜在选择剂。在该培养基中对53株铜绿假单胞菌进行了恢复测试，并将结果与​​市售类似测试中的结果进行了比较。选择性培养基，即假单胞菌琼脂和假单胞菌琼脂。这些比较的结果表明，带有C-390的假单胞菌琼脂F的抑制作用明显小于假单胞菌琼脂和假单胞菌分离的琼脂，并且比假单胞菌分离的琼脂更具选择性。 C-390在假单胞菌琼脂F中的掺入也提供了选择性和差异培养基。初步证据还表明，可以将C-390添加到其他基础培养基中，并获得可比的结果。

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