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首页> 外文期刊>Applied and Environmental Microbiology >Rapid latex agglutination test for detection of staphylococcal enterotoxins A to E that uses high-density latex particles.
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Rapid latex agglutination test for detection of staphylococcal enterotoxins A to E that uses high-density latex particles.

机译：快速乳胶凝集试验，用于检测使用高密度乳胶颗粒的葡萄球菌肠毒素A到E。

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摘要

A rapid reversed passive latex agglutination method that uses high-density latex particles for the detection of staphylococcal enterotoxins (SE) A to E was developed. It took 3 h for incubation, much less than the 16 h needed with a customary latex agglutination test for SE detection such as a commercial test kit (SET-RPLA; Denka Seiken Co. Ltd., Tokyo, Japan). The rapid test was shown to be highly specific and sensitive for SE detection (detection limit, about 0.5 ng of SE per ml), comparable to the SET-RPLA test. The rapid test was also efficient in SE detection in foods and culture supernatants of staphylococcal strains, similar to the SET-RPLA test. This showed that a rapid test with high-density latex particles is fully reliable for use.
机译：开发了一种快速反向被动胶乳凝集方法，该方法使用高密度胶乳颗粒检测葡萄球菌肠毒素（SE）A至E。孵育时间为3小时，远远少于用于SE检测的常规乳胶凝集测试（例如商业测试套件（SET-RPLA; Denka Seiken Co. Ltd.，东京，日本）所需的16小时）。快速测试显示对SE检测具有高度特异性和敏感性（检测限，每毫升约0.5 ng SE），与SET-RPLA测试相当。与SET-RPLA测试类似，快速测试在葡萄球菌菌株的食品和培养上清液中的SE检测中也很有效。这表明使用高密度乳胶颗粒进行的快速测试是完全可靠的。

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