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首页> 外文期刊>Applied and Environmental Microbiology >Effects of Polychlorinated Biphenyls on Macromolecular Synthesis by a Heterotrophic Marine Bacterium
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Effects of Polychlorinated Biphenyls on Macromolecular Synthesis by a Heterotrophic Marine Bacterium

机译：多氯联苯对异养海洋细菌大分子合成的影响

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Growth rates and final cell yields of a polychlorinated biphenyl (PCB)-sensitive pseudomonad isolated from the open ocean were reduced in a dose-dependent manner by 10 to 100 μg of Aroclor 1254 per liter, a commercial mixture of PCB isomers added to its culture medium. Effects on growth rates were detected within 1 h (approximately one doubling time) of treatment. By 4 h posttreatment, the amounts of deoxyribonucleic acid and ribonucleic acid per cell in exponentially growing populations treated with sublethal doses of Aroclor were detectably lower than in appropriate controls. Corresponding cell protein values were slightly higher than in controls. Selective degradation of cell proteins or nucleic acids was not detected in cells whose growth was totally suppressed for 4 h by PCBs. Cells whose growth rate was inhibited 20 to 50% by Aroclor synthesized protein at normal rates for periods in excess of 5 h from the time the chlorinated hydrocarbons were added. In contrast, rates per cell of adenine uptake and adenine incorporation into deoxyribonucleic acid and total nucleic acids by the cells treated with PCBs were significantly lower than in control cells. Intracellular adenine pools of cells whose growth was inhibited to 20% of the control rate by PCBs were 30% smaller and appeared to require a longer interval to equilibrate than those of untreated cells. This may indicate impaired transport and/or efflux of this nucleic acid precursor through the membrane of affected cells. Inhibition of nucleic acid synthesis in this sensitive bacterium by PCBs could explain the observed inhibitory effects of the chlorinated hydrocarbons on its growth.
机译：从公海分离的对多氯联苯（PCB）敏感的假单胞菌的生长速率和最终细胞产量以剂量依赖性方式降低每升10至100μgAroclor 1254，这是一种添加了PCB异构体的商业混合物，中。在治疗的1小时（约一倍的时间）内检测到对生长速率的影响。到处理后4小时，在亚致死剂量的Aroclor处理下，呈指数增长的群体中，每个细胞中的脱氧核糖核酸和核糖核酸的量明显低于适当的对照。相应的细胞蛋白值略高于对照组。在PCBs完全抑制其生长4小时的细胞中，未检测到细胞蛋白质或核酸的选择性降解。从加入氯化碳氢化合物开始，经过Aroclor合成蛋白以正常速率抑制了20至50％生长的细胞超过5小时。相反，用多氯联苯处理的细胞每细胞腺嘌呤摄取和腺嘌呤掺入脱氧核糖核酸和总核酸的比率显着低于对照细胞。多氯联苯将其生长抑制至控制率的20％的细胞内腺嘌呤池比未处理的细胞小30％，并且似乎需要更长的时间间隔才能达到平衡。这可能表明该核酸前体通过受影响的细胞膜的运输和/或流出受损。多氯联苯对这种敏感细菌的核酸合成抑制作用可以解释所观察到的氯代烃对其生长的抑制作用。

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