Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods.

A K Bej; M H Mahbubani; R M Atlas

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Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods.

机译：聚合酶链反应和基因探针法检测水中存活的肺炎军团菌。

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Methods using polymerase chain reaction (PCR) and gene probes to detect viable Legionella pneumophila were investigated with cells exposed to biocide or elevated temperature. Exposure to hypochlorite caused viable nonculturable cells to form. Culturable and viable nonculturable cells showed positive PCR amplification, whereas nonviable cells did not. Viable cells were also specifically detected with mip mRNA as the target, reverse transcription (to form cDNA), and PCR amplification. After exposure to elevated temperature, only viable culturable cells were detected, which corresponded with positive PCR amplification.

机译：在暴露于杀生物剂或高温的细胞中，研究了使用聚合酶链反应（PCR）和基因探针检测存活的军团杆菌的方法。暴露于次氯酸盐导致存活的不可培养细胞形成。可培养和存活的不可培养细胞显示出阳性PCR扩增，而不可存活的细胞则没有。还以mip mRNA为靶标，逆转录（形成cDNA）和PCR扩增特异性检测了活细胞。暴露于高温后，仅检测到可培养的活细胞，这与阳性PCR扩增相对应。

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《Applied and Environmental Microbiology》 |1991年第2期|共4页
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A K Bej; M H Mahbubani; R M Atlas;
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6. Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods. [O] . A K Bej, M H Mahbubani, R M Atlas 1991

机译：聚合酶链反应和基因探针法检测水中存活的肺炎军团菌。
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Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods.

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