Cloning and expression of a Bacteroides succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene in Escherichia coli.

J E Irvin; R M Teather

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Cloning and expression of a Bacteroides succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene in Escherichia coli.

机译：细菌拟杆菌琥珀酸混合链β-葡聚糖酶（1,3-1,4-β-D-葡聚糖4-葡糖酸水解酶）基因的克隆和表达。

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A pseudorandom genomic library of Bacteroides succinogenes DNA, cloned into pUC8 in Escherichia coli, was screened for beta-glucanase activity on 0.1% lichenan plates. Six high-activity clones, containing identical 5.2-kilobase inserts of B. succinogenes DNA, were obtained. The clones exhibited activity solely on beta-glucan substrates containing beta-(1----3)(1----4) linkages, thus manifesting a specific fibrolytic enzyme previously unrecognized in B. succinogenes. A subclone (pJI10) of the original insert (1.35 kilobases in size) expressed full beta-glucanase activity under control of its own promoter. The expression of beta-glucanase in pJI10 appeared subject to catabolite regulation by glucose. Detailed analysis of enzyme activity in the parental and deleted derivatives, subcloned into pUC18 and pUC19, suggested that the apparent glucose repression was an artifact arising as a consequence of interactions with the lac transcriptional unit in the plasmid vector.

机译：克隆到大肠杆菌中的pUC8中的拟杆菌琥珀酸杆菌DNA的伪随机基因组文库在0.1％地衣聚糖板上筛选了β-葡聚糖酶活性。获得了六个高活性克隆，其中含有相同的琥珀酸芽孢杆菌DNA的5.2碱基碱基插入片段。这些克隆仅对含有β-（1–3）（1 ---- 4）键的β-葡聚糖底物表现出活性，因此表现出一种以前在琥珀酸芽孢杆菌中未被发现的特定纤溶酶。原始插入片段（大小为1.35千碱基）的亚克隆（pJI10）在其自身启动子的控制下表达了完整的β-葡聚糖酶活性。 β-葡聚糖酶在pJI10中的表达似乎受葡萄糖的分解代谢调节。对亚克隆到pUC18和pUC19中的亲本和缺失的衍生物中酶活性的详细分析表明，表观的葡萄糖抑制是由于与质粒载体中lac转录单位相互作用而产生的假象。

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《Applied and Environmental Microbiology》 |1988年第11期|共5页
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J E Irvin; R M Teather;
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1. Cloning and expression of a Bacteroides succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene in Escherichia coli. [J] . J E Irvin, R M Teather Applied and Environmental Microbiology . 1988,第11期

机译：细菌拟杆菌琥珀酸混合链β-葡聚糖酶（1,3-1,4-β-D-葡聚糖4-葡糖酸水解酶）基因的克隆和表达。
2. DNA sequence of a Fibrobacter succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene. [J] . R M Teather, J D Erfle Journal of bacteriology . 1990,第7期

机译：琥珀酸纤维杆菌混合链接β-葡聚糖酶（1,3-1,4-β-D-葡聚糖4-葡糖酸水解酶）基因的DNA序列。
3. Purification and properties of a 1,3-1,4-β-d-glucanase (lichenase, 1,3-1,4-β-d-glucan 4-glucanohydrolase, EC 3.2.1.73) from Bacteroides succinogenes cloned in Escherichia coli [J] . J D Erfle, J E Irvin, P J Wood, The biochemical journal . 1988,第3期

机译：从克隆到大肠杆菌中的琥珀酸杆菌的1,3-1,4-β-d-葡聚糖酶（地衣聚糖酶，1,3-1,4-β-d-葡聚糖4-葡糖酸水解酶，EC 3.2.1.73）的纯化和特性
4. BRIEF COMMUNICATION: Expression of innate immune response genes in mammary epithelia following stimulation with lipopolysaecharide or Escherichia coli. [C] . C. STOCKUM, B.J. HAIGH, H.H.D. MEYER, Conference of the New Zealand Society of Animal Production . 2008

机译：简介：用脂多利亚替代术或大肠杆菌刺激后乳腺上皮内先天免疫应答基因的表达。
5. Heterodimeric deoxyguanosine/deoxyadenosine kinase from Lactobacillus acidophilus R-26: Affinity protein purification, molecular cloning, sequence of the genes, and expression in Escherichia coli. [D] . Ma, Grace Tak-Yi. 1993

机译：嗜酸乳杆菌R-26的异二聚体脱氧鸟苷/脱氧腺苷激酶：亲和蛋白纯化，分子克隆，基因序列和在大肠杆菌中的表达。
6. Cloning and expression of a Bacteroides succinogenes mixed-linkage beta-glucanase (13-14-beta-D-glucan 4-glucanohydrolase) gene in Escherichia coli. [O] . J E Irvin, R M Teather 1988

机译：拟杆菌的琥珀酸混合链β-葡聚糖酶（13-14-β-D-D-葡聚糖4-葡聚糖水解酶）基因的克隆和表达在大肠杆菌中。
7. DNA sequence of a Fibrobacter succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene. [O] . Teather, R M, Erfle, J D 1990

机译：琥珀酸纤维杆菌混合链接的β-葡聚糖酶（1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶）基因的DNA序列。
8. Transfer of 'Bacteroides succinogenes' (Hungate) to 'Fibrobacter' gen. nov. as 'Fibrobacter succinogenes' comb. nov. and Description of 'Fibrobacter intestinalis' sp. nov [R] . Montgomery, L. , Flesher, B. , Stahl, D. 1988

机译：将'Bacteroides succinogenes'（Hungate）转移到'Fibrobacter'基因。十一月作为'Fibrobacter succinogenes'梳子。十一月和'Fibrobacter intestinalis'sp。的描述十一月

Cloning and expression of a Bacteroides succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene in Escherichia coli.

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