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首页> 外文期刊>Applied and Environmental Microbiology >Characterization of Peroxidase Secretion and Subcellular Organization of Phanerochaete chrysosporium INA-12 in the Presence of Various Soybean Phospholipid Fractions
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Characterization of Peroxidase Secretion and Subcellular Organization of Phanerochaete chrysosporium INA-12 in the Presence of Various Soybean Phospholipid Fractions

机译：大豆磷脂组分存在下Phanerochaete chrysosporium INA-12过氧化物酶分泌和亚细胞结构的表征

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Stimulation of lignin peroxidase production by exogenous phospholipids depends on the composition of the phospholipid fraction prepared by using the Nattermann process. The fraction composed mainly of negatively charged phospholipids (NAT 89) was the most efficient source for exoprotein secretion by Phanerochaete chrysosporium INA-12. The results of biochemical marker assays and ultrastructural morphology determination by electron microscopy were correlated. Activities of succinate dehydrogenase, a mitochondrial marker, and cytochrome c oxidoreductase, an endoplasmic reticulum (ER) marker, were increased 1.3- and 2.2-fold, respectively, in the presence of NAT 89. Electron microscopy observations suggested that the amount of mitochondria and ER in culture containing phospholipids was increased at the optimum day of lignin peroxidase production. Therefore, phospholipids enhanced energetic metabolism of strain INA-12 and markedly modified fungus physiology. Since ER is involved in enzyme synthesis, we suggest that its increased amount in mycelium cultured with NAT 89 is directly associated with the higher production of lignin peroxidase.
机译：外源性磷脂刺激木质素过氧化物酶产生取决于通过使用Nattermann法制备的磷脂级分的组成。主要由带负电荷的磷脂（NAT 89）组成的部分是Phanerochaete chrysosporium INA-12分泌外蛋白最有效的来源。将生化标记物测定结果和电子显微镜测定的超微结构形态相关联。在存在NAT 89的情况下，线粒体标记物琥珀酸脱氢酶和内质网（ER）标记细胞色素c氧化还原酶的活性分别增加了1.3倍和2.2倍。电子显微镜观察表明，线粒体和线粒体的量在木质素过氧化物酶产生的最佳天，含有磷脂的培养物中的ER增加。因此，磷脂增强了INA-12菌株的能量代谢，并显着改变了真菌的生理功能。由于ER参与酶的合成，我们建议用NAT 89培养的菌丝体中ER的增加与木质素过氧化物酶的更高产量直接相关。

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