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首页> 外文期刊>Applied and Environmental Microbiology >Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophile 'Caldocellum saccharolyticum'.
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Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophile 'Caldocellum saccharolyticum'.

机译：嗜热菌“解糖卡尔德氏菌”编码木聚糖降解酶的基因的克隆，序列分析和表达。

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摘要

A lambda recombinant bacteriophage coding for xylanase and beta-xylosidase activity has been isolated from a genomic library of the extremely thermophilic anaerobe "Caldocellum saccharolyticum." Partial Sau3AI fragments of the lambda recombinant DNA were ligated into pBR322. A recombinant plasmid with an insertion of ca. 7 kilobases of thermophilic DNA expressing both enzymatic activities was isolated. The location of the genes has been established by analyzing deletion derivatives, and the DNA sequence of 6.067 kilobases of the insert has been determined. Five open reading frames (ORFs) were found, one of which (ORF1; Mr 40,455) appears to code for a xylanase (XynA) which also acts on o-nitrophenyl-beta-D-xylopyranoside. Another, ORF5 (Mr 56,365), codes for a beta-xylosidase (XynB). The xynA gene product shows significant homology with the xylanases from the alkalophilic Bacillus sp. strain C125 and Clostridium thermocellum.
机译：从极端嗜热的厌氧菌“解藻卡尔德氏菌”的基因组文库中分离出编码木聚糖酶和β-木糖苷酶活性的λ重组噬菌体。将λ重组DNA的部分Sau3AI片段连接到pBR322中。插入有ca.1的重组质粒。分离出表达两种酶活性的7kb的嗜热DNA。通过分析缺失衍生物确定了基因的位置，并确定了插入片段的6.067千碱基的DNA序列。发现了五个开放阅读框（ORF），其中一个（ORF1; Mr 40,455）似乎编码木聚糖酶（XynA），该酶也作用于邻硝基苯基-β-D-吡喃吡喃糖苷。另一个是ORF5（56,365先生），编码β-木糖苷酶（XynB）。 xynA基因产物与来自嗜碱芽孢杆菌属的木聚糖酶显示出显着的同源性。菌株C125和热纤梭菌。

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