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首页> 外文期刊>Applied and Environmental Microbiology >Ribotyping to differentiate Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses.
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Ribotyping to differentiate Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses.

机译：核型分型以区分坏死镰刀菌亚种。死灵藻和死灵藻亚种。从牛瘤胃内容物和肝脓肿中分离出的胃底。

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Differences in biological activities (hemagglutination, hemolytic, leukotoxic, and virulence) and ribotypes between the two subspecies of Fusobacterium necrophorum of bovine ruminal and liver abscess origins were investigated. Hemagglutination activity was present in all hepatic, but only some ruminal, strains of Fusobacterium necrophorum subsp. necrophorum. Ruminal F. necrophorum subsp. necrophorum had low leukotoxin titers yet was virulent in mice. Fusobacterium necrophorum subsp. funduliforme of hepatic or ruminal origin had no hemagglutination activity, had low hemolytic and leukotoxic activities, and was less virulent to mice. For ribotyping, chromosomal DNAs of 10 F. necrophorum subsp. necrophorum and 11 F. necrophorum subsp. funduliforme isolates were digested with restriction endonucleases (EcoRI, EcoRV, SalI, PstI, and HaeIII) and examined by restriction fragment length polymorphisms after hybridizing with a digoxigenin-labeled cDNA probe transcribed from a mixture of 16 and 23S rRNAs from Escherichia coli. The most discriminating restriction endonuclease enzyme for ribotyping was EcoRI. The presence or absence of two distinct bands of 2.6 and 4.3 kb differentiated the two subspecies. Regardless of the origin, only F. necrophorum subsp. necrophorum, a virulent subspecies, had a ca. 2.6-kb band, whereas F. necrophorum subsp. funduliforme, a less virulent subspecies, had a ca. 4.3-kb band. Ribotyping appears to be a useful technique to genetically differentiate the two subspecies of F. necrophorum.
机译：研究了牛瘤胃和猪脓肿的坏死镰刀菌两个亚种之间的生物学活性（血凝，溶血，白细胞毒性和毒力）和核糖型的差异。血凝活性存在于所有肝脏中，但仅一些瘤胃坏死杆菌亚种的瘤胃菌株。死灵瘤胃坏​​死菌亚种。死灵具有较低的白细胞毒素滴度，但对小鼠却有毒。坏死镰刀菌亚种。肝或瘤胃来源的眼底无血凝活性，溶血和白细胞毒性低，对小鼠的毒性较小。对于核糖分型，10 F. necrophorum亚种的染色体DNA。 necrophorum和11 F. necrophorum亚种。用限制性核酸内切酶（EcoRI，EcoRV，SalI，PstI和HaeIII）消化眼底目分离物，并与从大肠杆菌中16和23S rRNA的混合物转录得到的洋地黄毒苷标记的cDNA探针杂交后，通过限制性片段长度多态性进行检查。用于核糖分型的最有区别的限制性核酸内切酶是EcoRI。 2.6和4.3 kb两个不同条带的存在与否将这两个亚种区分开来。不论起源如何，仅镰孢镰刀菌亚种。坏死性坏死菌（necrophorum）有一个约。 2.6-kb波段，而死镰孢亚种胃底亚种，一种毒性较小的亚种，大约有一个。 4.3 kb频段。核型分型似乎是从遗传上区分镰刀菌两个亚种的有用技术。

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