Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization.

A R Datta; M A Moore; B A Wentz; J Lane

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Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization.

机译：非放射性DNA探针菌落杂交鉴定和计数单核细胞增生李斯特菌。

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A plasmid containing the cloned listeriolysin gene of Listeria monocytogenes was used as a probe to identify Listeria strains by DNA colony hybridization. The probe DNA was labeled with horseradish peroxidase in the presence of glutaraldehyde. After the hybridization and wash procedures, the hybrid molecules were detected by luminescence, which resulted from the oxidation of luminol by a horseradish peroxidase-hydrogen peroxide-coupled reaction. Of the 150 Listeria strains and 16 non-Listeria strains examined, the probe hybridized only with L. monocytogenes. The technique was also used to enumerate L. monocytogenes in artificially contaminated foods.

机译：使用含有克隆的单核细胞增生李斯特菌的李斯特菌溶血素基因的质粒作为探针，通过DNA菌落杂交鉴定李斯特菌。在戊二醛存在下，用辣根过氧化物酶标记探针DNA。杂交和洗涤程序后，通过发光检测杂合分子，这是由于辣根过氧化物酶-过氧化氢偶合反应使鲁米诺氧化而产生的。在150个李斯特菌菌株和16个非李斯特菌菌株中，探针仅与单核细胞增生李斯特菌杂交。该技术还用于枚举人工污染食品中的单核细胞增生李斯特菌。

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《Applied and Environmental Microbiology》 |1993年第1期|共6页
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A R Datta; M A Moore; B A Wentz; J Lane;
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中图分类植物学;
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1. Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization. [J] . A R Datta, M A Moore, B A Wentz, Applied and Environmental Microbiology . 1993,第1期

机译：非放射性DNA探针菌落杂交鉴定和计数单核细胞增生李斯特菌。
2. Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization. [J] . A R Datta, B A Wentz, W E Hill Applied and Environmental Microbiology . 1987,第9期

机译：通过使用DNA菌落杂交检测溶血性单核细胞增生性李斯特菌。
3. Identification of enterotoxigenic Escherichia coli by colony hybridization with nonradioactive digoxigenin-labeled DNA probes. [J] . L K Riley, C J Caffrey Journal of Clinical Microbiology . 1990,第6期

机译：通过用非放射性洋地黄毒苷标记的DNA探针进行菌落杂交来鉴定产肠毒素的大肠杆菌。
4. Listeria monocytogenes Colony Counting from Microscopic Images Using Nonlinear Piecewise Least-Square Curve-Fitting Filter [C] . Pahnchawatt, Kitthichai, Lipikorn, International Conference on Information Science and Applications . 2013

机译：使用非线性分段最小二乘曲线拟合滤波器从显微图像计数单核细胞增生李斯特菌
5. Listeria monocytogenes: Isolation methods, resistance to the lactoperoxidase antimicrobial system, occurrence of petite colonies, and production of monoclonal antibodies. [D] . Siragusa, Gregory Ross. 1989

机译：单核细胞增生性李斯特菌：分离方法，对乳过氧化物酶抗微生物系统的抗性，小的集落的出现以及单克隆抗体的产生。
6. Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization. [O] . A R Datta, M A Moore, B A Wentz, 1993

机译：非放射性DNA探针菌落杂交鉴定和计数单核细胞增生李斯特菌。
7. Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization [O] . A R Datta, M A Moore, B A Wentz, 1993

机译：非酰基活性DNA探针菌落杂交李斯特菌单核细胞增生的鉴定及调查

Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization.

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