Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.

A R Datta; B A Wentz; W E Hill

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Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.

机译：通过使用DNA菌落杂交检测溶血性单核细胞增生性李斯特菌。

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A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.

机译：单核细胞增生李斯特氏菌的约500个碱基对的β-溶血素基因片段被用于通过DNA菌落杂交筛选不同的细菌菌株。在氢氧化钠存在下，用微波裂解菌落中的细胞。在筛选的52种李斯特菌不同菌株中，仅单核细胞增生李斯特氏菌的β-溶血（CAMP阳性）菌株的DNA与该探针杂交。

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《Applied and Environmental Microbiology》 |1987年第9期|共4页
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A R Datta; B A Wentz; W E Hill;
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中图分类植物学;
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专利

1. Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization. [J] . A R Datta, M A Moore, B A Wentz, Applied and Environmental Microbiology . 1993,第1期

机译：非放射性DNA探针菌落杂交鉴定和计数单核细胞增生李斯特菌。
2. Identification and enumeration of Listeria monocytogenes by nonradioactive DNA probe colony hybridization. [J] . A R Datta, M A Moore, B A Wentz, Applied and Environmental Microbiology . 1993,第1期

机译：非放射性DNA探针菌落杂交鉴定和计数单核细胞增生李斯特菌。
3. Detection of Listeria monocytogenes by direct colony hybridization on hydrophobic grid-membrane filters by using a chromogen-labeled DNA probe. [J] . P I Peterkin, E S Idziak, A N Sharpe Applied and Environmental Microbiology . 1991,第2期

机译：通过使用发色团标记的DNA探针在疏水性网格膜滤膜上直接菌落杂交来检测单核细胞增生李斯特菌。
4. Detection of hlyA gene of Listeria monocytogenes with Electrochemical DNA Biosensor [C] . Lingwei Wu, Quanjun Liu, Zhongwei Wu, . 2008

机译：电化学DNA生物传感器检测单核细胞增生李斯特菌hlyA基因
5. Development of a mariner based transposon and identification of Listeria monocytogenes determinants, including the peptidyl-prolyl isomerase PrsA2, that contribute to its hemolytic phenotype. [D] . Zemansky, Jason Eron. 2009

机译：基于水手的转座子的开发和李斯特菌单核细胞增生因子决定簇的鉴定，包括有助于其溶血表型的肽基-脯氨酰异构酶PrsA2。
6. Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization. [O] . A R Datta, B A Wentz, W E Hill 1987

机译：使用DNA菌落杂交检测溶血性单核细胞增生性李斯特菌。
7. Detection of Listeria monocytogenes by direct colony hybridization on hydrophobic grid-membrane filters by using a chromogen-labeled DNA probe [O] . P I Peterkin, E S Idziak, A N Sharpe 1991

机译：用色原标记的DNA探针通过直接菌落杂交在疏水桥膜过滤器上的李斯特菌单核细胞增生检测

Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.

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