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首页> 外文期刊>Applied and Environmental Microbiology >Characterization of a new pathway for epichlorohydrin degradation by whole cells of xanthobacter strain py2.
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Characterization of a new pathway for epichlorohydrin degradation by whole cells of xanthobacter strain py2.

机译：Xanthobacter菌株py2的全细胞降解表氯醇的新途径的表征。

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The degradation of epichlorohydrin (3-chloropropylene oxide or 1-chloro-2,3-epoxypropane) by whole-cell suspensions of Xanthobacter strain Py2 was investigated. Cell suspensions prepared from cultures grown with propylene as the carbon source readily degraded epichlorohydrin. The ability to degrade epichlorohydrin correlated with the expression of enzymes involved in alkene and epoxide metabolism, since cell suspensions prepared from cultures grown with glucose or acetone, in which the enzymes of alkene and epoxide oxidation are not expressed, did not degrade epichlorohydrin. The alkene monooxygenase-specific inhibitor propyne had no effect on the degradation of epichlorohydrin, demonstrating that alkene monooxygenase is not involved in epichlorohydrin conversion. The interaction of epichlorohydrin and epibromohydrin with the epoxidase which catalyzes aliphatic epoxide conversions was established by showing that the epihalohydrins were specific and potent inhibitors of propylene oxide-dependent O(inf2) consumption by cell suspensions. The rates of degradation of epoxides in whole-cell suspensions decreased in the series propylene oxide > epifluorohydrin > epichlorohydrin > epibromohydrin. The pathway of epichlorohydrin degradation was investigated and found to proceed with stoichiometric dechlorination of epichlorohydrin. The first detectable product of epichlorohydrin degradation was chloroacetone. Chloroacetone was further degraded by the cell suspensions, and in the process, acetone was formed as a nonstoichiometric product. Acetone was further degraded by the cell suspensions with enzymes apparently induced by the accumulation of acetone. The metabolism of allyl chloride (3-chloropropylene) by propylene-grown cells was initiated by alkene monooxygenase and proceeded through epichlorohydrin, chloroacetone, and acetone as intermediate degradation products. These studies reveal a new pathway for halogenated epoxide degradation which involves halogenated and aliphatic ketones as well as other unidentified intermediates and which is unique from previously characterized hydrolytic degradative pathways.
机译：研究了黄杆菌属菌株Py2的全细胞悬浮液对表氯醇（3-氯环氧丙烷或1-氯-2,3-环氧丙烷）的降解作用。由以丙烯作为碳源生长的培养物制备的细胞悬液易于降解表氯醇。降解表氯醇的能力与参与烯烃和环氧化物代谢的酶的表达相关，因为从葡萄糖或丙酮生长的培养物中制备的细胞悬液不会降解表氯醇，其中葡萄糖或丙酮不表达烯烃和环氧化物的酶。烯烃单加氧酶特异性抑制剂丙炔对表氯醇的降解没有影响，表明烯烃单加氧酶不参与表氯醇的转化。表氯醇和表溴醇与催化脂肪族环氧化物转化的环氧酶的相互作用是通过证明表卤代醇是细胞悬浮液对环氧丙烷依赖性O（inf2）消耗的特异性和强效抑制剂而建立的。在环氧丙烷>表氟醇>表氯醇>表溴醇系列中，全细胞悬浮液中环氧化物的降解速率降低。研究了环氧氯丙烷降解的途径，发现环氧氯丙烷的化学计量脱氯是继续进行的。表氯醇降解的第一个可检测产物是氯丙酮。氯丙酮被细胞悬浮液进一步降解，在此过程中，丙酮以非化学计量形式生成。丙酮被细胞悬浮液进一步降解，其中的酶显然是由丙酮的积累诱导的。丙烯生长的细胞对烯丙基氯（3-氯丙烯）的代谢是由烯烃单加氧酶引发的，并通过表氯醇，氯丙酮和丙酮作为中间降解产物进行。这些研究揭示了卤代环氧化物降解的新途径，该途径涉及卤代和脂肪族酮以及其他未鉴定的中间体，这是以前表征的水解降解途径所独有的。

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