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首页> 外文期刊>Applied and Environmental Microbiology >TOM, a new aromatic degradative plasmid from Burkholderia (Pseudomonas) cepacia G4.
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TOM, a new aromatic degradative plasmid from Burkholderia (Pseudomonas) cepacia G4.

机译：TOM，一种新的伯克霍尔德氏菌（假单胞菌）洋葱G4的芳香族降解质粒。

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Burkholderia (Pseudomonas) cepacia PR1(23) has been shown to constitutively express to toluene catabolic pathway distinguished by a unique toluene ortho-monooxygenase (Tom). This strain has also been shown to contain two extrachromosomal elements of 100 kb. A derivative strain cured of the largest plasmid, PR1(23) Cure, was unable to grow on phenol or toluene as the sole source of carbon and energy, which requires expression of the Tom pathway. Transfer of the larger plasmid from strain G4 (the parent strain inducible for Tom) enabled PR1(23) Cure to grow on toluene or phenol via inducible Tom pathway expression. Conjugal transfer of TOM23c from PR1(23) to an antibiotic-resistant derivative of PR1(23) Cure enabled the transconjugant to grow with either phenol or toluene as the sole source of carbon and energy through constitutive expression of the Tom pathway. A cloned 11.2-kb EcoRI restriction fragment of TOM23c resulted in the expression of both Tom and catechol 2,3-dioxygenase in Escherichia coli, as evidenced by its ability to oxidize trichloroethylene, toluene, m-cresol, o-cresol, phenol, and catechol. The largest resident plasmid of PR1 was identified as the source of these genes by DNA hybridization. These results indicate that the genes which encode Tom and catechol 2,3-dioxygenase are located on TOM, an approximately 108-kb degradative plasmid of B. cepacia G4.
机译：Burkholderia（Pseudomonas）cepacia PR1（23）已被证明可组成性表达以独特的甲苯原单加氧酶（Tom）区分的甲苯分解代谢途径。该菌株还显示含有两个100 kb的染色体外元件。用最大质粒PR1（23）Cure固化的衍生菌株无法在苯酚或甲苯上生长，因为碳和能量是唯一的碳和能量来源，因此需要表达Tom途径。从菌株G4（Tom可诱导的亲本菌株）中转移更大的质粒，使PR1（23）Cure通过可诱导的Tom途径表达在甲苯或苯酚上生长。 TOM23c从PR1（23）到PR1（23）的抗生素抗性衍生物的共轭转移Cure使该共轭结合物能够与Tom的组成型表达一起与苯酚或甲苯作为碳和能量的唯一来源一起生长。克隆的TOM23c的11.2kb EcoRI限制性片段可导致Tom和邻苯二酚2,3-二加氧酶在大肠杆菌中表达，这可由其氧化三氯乙烯，甲苯，间甲酚，邻甲酚，苯酚和儿茶酚。通过DNA杂交鉴定出PR1最大的驻留质粒是这些基因的来源。这些结果表明，编码Tom和邻苯二酚2，3-二加氧酶的基因位于TOM上，即洋葱双歧杆菌G4的大约108kb的降解质粒。

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