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首页> 外文期刊>Applied and Environmental Microbiology >Solution (sup13)C Nuclear Magnetic Resonance Spectroscopic Analysis of the Amino Acids of Methanosphaera stadtmanae: Biosynthesis and Origin of One-Carbon Units from Acetate and Carbon Dioxide.
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Solution (sup13)C Nuclear Magnetic Resonance Spectroscopic Analysis of the Amino Acids of Methanosphaera stadtmanae: Biosynthesis and Origin of One-Carbon Units from Acetate and Carbon Dioxide.

机译：斯坦甲烷甲烷八叠球菌的氨基酸的溶液（sup13）C核磁共振波谱分析：乙酸和二氧化碳中一碳单元的生物合成和来源。

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We found that general pathways for amino acid synthesis of Methanosphaera stadtmanae, a methanogen that forms CH(inf4) from H(inf2) and methanol, resembled those of methanogens that form CH(inf4) from CO(inf2) or from the methyl group of acetate. We determined the incorporation of (sup14)C-labeled CO(inf2), formate, methanol, methionine, serine, and acetate into cell macromolecules. Labeling of amino acid carbons was determined by solution nuclear magnetic resonance spectroscopy after growth with (sup13)C-labeled acetate, CO(inf2), serine, and methanol. The (alpha) and (beta) carbons of serine and alanine were formed from carboxyl and methyl carbons of acetate, respectively, and the amino acid carboxyl groups were formed from CO(inf2). This indicates that pyruvate was formed by reductive carboxylation of acetate. Labeling of the methyl carbon of methionine indicated that the major route of synthesis was from the hydroxymethyl carbon of serine that arises from the methyl carbon of acetate. Methanol was a minor source of the methyl of methionine. Unambiguous assignment was made of the sources of all carbons of histidine. Labeling of the histidine 7 position ((epsilon) carbon) was consistent with formation from the C-2 of the purine ring of ATP and the origin of the C-2 from a formyl unit derived from the hydroxymethyl carbon of serine.
机译：我们发现，甲烷甲烷八叠球菌的氨基酸合成的一般途径是由H（inf2）和甲醇形成CH（inf4）的产甲烷菌，类似于由CO（inf2）或由甲基的甲基形成CH（inf4）的产甲烷菌。醋酸盐。我们确定将（sup14）C标记的CO（inf2），甲酸盐，甲醇，蛋氨酸，丝氨酸和乙酸盐掺入细胞大分子中。用（sup13）C标记的乙酸盐，CO（inf2），丝氨酸和甲醇生长后，通过溶液核磁共振波谱法测定氨基酸碳的标记。丝氨酸和丙氨酸的α和β碳分别由乙酸的羧基和甲基形成，氨基酸羧基由CO（inf2）形成。这表明丙酮酸酯是由乙酸的还原羧化形成的。蛋氨酸甲基碳的标记表明合成的主要途径是来自丝氨酸的羟甲基碳，而丝氨酸的羟甲基碳则来自乙酸甲酯。甲醇是蛋氨酸甲基的次要来源。对组氨酸所有碳的来源进行了明确分配。组氨酸7位（（ε）碳）的标记与ATP嘌呤环的C-2的形成以及C-2源自丝氨酸羟甲基碳的甲酰基单元的形成一致。

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