...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied and Environmental Microbiology >Antibiotic Resistance Patterns of Enterococci and Occurrence of Vancomycin-Resistant Enterococci in Raw Minced Beef and Pork in Germany
【24h】

Antibiotic Resistance Patterns of Enterococci and Occurrence of Vancomycin-Resistant Enterococci in Raw Minced Beef and Pork in Germany

机译：德国生牛肉和猪肉中肠球菌的抗生素耐药性模式和耐万古霉素肠球菌的发生

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The food chain, especially raw minced meat, is thought to be responsible for an increase in the incidence of vancomycin-resistant enterococci (VRE) in human nosocomial infections. Therefore, 555 samples from 115 batches of minced beef and pork from a European Union-licensed meat-processing plant were screened for the occurrence of VRE. The processed meat came from 45 different slaughterhouses in Germany. Enterococci were isolated directly from Enterococcosel selective agar plates and also from Enterococcosel selective agar plates supplemented with 32 mg of vancomycin per liter. In addition, peptone broth was used in a preenrichment procedure, and samples were subsequently plated onto Enterococcosel agar containing vancomycin. To determine resistance, 209 isolates from 275 samples were tested with the glycopeptides vancomycin, teicoplanin, and avoparcin and 19 other antimicrobial substances by using a broth microdilution test. When the direct method was used, VRE were found in 3 of 555 samples (0.5%) at a concentration of 1.0 log CFU/g of minced meat. When the preenrichment procedure was used, 8% of the samples were VRE positive. Our findings indicate that there is a low incidence of VRE in minced meat in Germany. In addition, the resistance patterns of the VRE isolates obtained were different from the resistance patterns of clinical isolates. A connection between the occurrence of VRE in minced meat and nosocomial infections could not be demonstrated on the basis of our findings.
机译：人们认为，食物链，尤其是生碎肉，是导致人类医院感染中耐万古霉素肠球菌（VRE）发生率增加的原因。因此，对来自欧盟许可的肉类加工厂的115批次牛肉和猪肉碎中的555个样品进行了VRE筛选。加工的肉来自德国的45个不同的屠宰场。肠球菌可直接从肠球菌选择性琼脂平板中分离，也可从每升补充有32 mg万古霉素的肠球菌选择性琼脂平板中分离。另外，在预富集程序中使用蛋白bro肉汤，随后将样品铺板到含有万古霉素的肠球菌琼脂上。为了确定抗药性，通过肉汤微量稀释试验，用糖肽万古霉素，替考拉宁和阿伏巴星以及其他19种抗微生物物质测试了275个样品中的209个分离株。当使用直接方法时，在555个样品中的3个（0.5％）中发现VRE，每克肉末的浓度为1.0 log CFU。当使用预富集程序时，有8％的样品为VRE阳性。我们的发现表明，德国碎肉中VRE的发生率较低。另外，获得的VRE分离株的抗性模式与临床分离株的抗性模式不同。根据我们的发现，肉糜中VRE的发生与医院感染之间没有联系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号