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首页> 外文期刊>Applied and Environmental Microbiology >Extent of Variation of the Bacillus thuringiensis Toxin Reservoir: the Case of the Geranium Bronze, Cacyreus marshalli Butler (Lepidoptera: Lycaenidae)
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Extent of Variation of the Bacillus thuringiensis Toxin Reservoir: the Case of the Geranium Bronze, Cacyreus marshalli Butler (Lepidoptera: Lycaenidae)

机译：苏云金芽孢杆菌毒素贮库的变异程度：以天竺葵青铜，马赛·巴塞勒（Cacyreus marshalli Butler）为例（鳞翅目：Lycaenidae）

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Despite the fact that around 200 cry genes from Bacillus thuringiensis have already been cloned, only a few Cry proteins are toxic towards a given pest. A crucial step in the mode of action of Cry proteins is binding to specific sites in the midgut of susceptible insects. Binding studies in insects that have developed cross-resistance discourage the combined use of Cry proteins sharing the same binding site. If resistance management strategies are to be implemented, the arsenal of Cry proteins suitable to control a given pest may be not so vast as it might seem at first. The present study evaluates the potential of B. thuringiensis for the control of a new pest, the geranium bronze (Cacyreus marshalli Butler), a butterfly that is threatening the popularity of geraniums in Spain. Eleven of the most common Cry proteins from the three lepidopteran-active Cry families (Cry1, Cry2, and Cry9) were tested against the geranium bronze for their toxicity and binding site relationships. Using 125I-labeled Cry1A proteins we found that, of the seven most active Cry proteins, six competed for binding to the same site. For the long-term control of the geranium bronze with B. thuringiensis-based insecticides it would be advisable to combine any of the Cry proteins sharing the binding site (preferably Cry1Ab, since it is the most toxic) with those not competing for the same site. Cry1Ba would be the best choice of these proteins, since it is significantly more toxic than the others not binding to the common site.
机译：尽管已经从苏云金芽孢杆菌中克隆了大约200个cry基因，但只有少数Cry蛋白对特定的有害生物具有毒性。 Cry蛋白作用方式的关键步骤是与易感昆虫中肠的特定位点结合。对已经产生交叉抗性的昆虫的结合研究不鼓励共同使用具有相同结合位点的Cry蛋白。如果要实施抗药性管理策略，那么适合控制特定害虫的Cry蛋白库可能并不像乍看起来那样庞大。本研究评估了苏云金芽孢杆菌控制一种新害虫天竺葵青铜（Cacyreus marshalli Butler）的潜力，这种蝴蝶威胁着西班牙天竺葵的流行。测试了来自三个鳞翅目活跃Cry家族（Cry1，Cry2和Cry9）中的11种最常见的Cry蛋白针对天竺葵青铜的毒性和结合位点的关系。我们使用125 I标记的Cry1A蛋白发现，在七个活性最高的Cry蛋白中，六个竞争与相同位点的结合。为了用苏云金芽孢杆菌为基础的杀虫剂长期控制天竺葵青铜，建议将共享结合位点的任何Cry蛋白（最好是Cry1Ab，因为它最具毒性）与不竞争它们的那些结合起来使用。现场。 Cry1Ba将是这些蛋白质的最佳选择，因为它比不结合共同位点的其他蛋白质具有更大的毒性。

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