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首页> 外文会议>International Conference on Environmental, Industrial and Applied Microbiology >The toxicity and histopathology of Bacillus thuringiensis Cry1Ba toxin to Spodoptera frugiperda (Lepidoptera, Noctuidae)
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The toxicity and histopathology of Bacillus thuringiensis Cry1Ba toxin to Spodoptera frugiperda (Lepidoptera, Noctuidae)

机译：芽孢杆菌Cry1ba毒素对烟草蛋白酶的毒性和组织病理学（Lepidoptera，Noctuidae）

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Agricultural pest insects have been controlled primarily through the use of chemical insecticides. Bacillus thuringiensis (Bt) bacterium is an alternative for biological control since it synthesizes entomopathogenic protoxins which are codified by different cry genes. This study evaluated the insecticidal activity and the histopathological effects of bacterial suspension and purified Cry1Ba protein from B. thuringiensis thuringiensis strain 4412 in the midgut epithelial cells of S. frugiperda larvae. To accomplish that, bioassays were done with bacteriological suspension of the Bt strain, which was applied to the Poitout diet, and the toxicity of this strain was also determined by the LC_(50), using purified protein, where the diet was substituted by disks of corn leaf. In the control group the protein and bacterial culture were replaced by sterile distilled water. S. frugiperda larvae of 2~(nd) instar were prepared through the inclusion paraffin techniques to evaluate the histopathology. Our results showed that the tested B. thuringiensis thuringiensis 4412, was highly toxic in the bioassays with 100% of corrected mortality to S. frugiperda larvae. In addition, the LC_(50) results indicated that the expressed Cry1Ba protein was toxic too, with a LC_(50) of 10.88 μg/ml. The histopathological analysis of S. frugiperda midgut showed a progressive loss of epithelial cell definition after 3h, in both treatments. At 24h post treatment, their midgut changes included vacuolization of the cytoplasm, hypertrophy of the epithelial cells, and vesicle formation in the apical region of both goblet and columnar cells. Also, the brush border membrane was damaged, especially in goblet cells.
机译：农业害虫昆虫主要通过使用化学杀虫剂来控制。芽孢杆菌（BT）细菌是生物控制的替代方案，因为它合成了由不同乳汁基因编纂的昆虫致病原料毒素。该研究评估了在S.Frugiperda幼虫的中间蛋白上皮细胞中的杀虫活性和纯化的Cry1Ba蛋白的杀虫活性和纯化的Cry1Ba蛋白的组织病理学作用。为了实现这个目标，生物测定与BT菌株，将其施加到Poitout饮食的细菌悬浮液进行，并且也通过LC_（50）来确定该菌株的毒性，使用纯化的蛋白质，其中所述饮食由磁盘取代玉米叶。在对照组中，蛋白质和细菌培养物被无菌蒸馏水取代。通过包含石蜡技术制备2〜（ND）绒毛的S.Fugiperda幼虫以评估组织病理学。我们的研究结果表明，Thuraliensis Thuringiensis 4412的测试B. Thuringiensis sthuringiensis 4412在生物测定中具有浓重的毒性，其较为矫正的死亡率为S.Frugiperda幼虫。另外，LC_（50）结果表明表达的Cry1Ba蛋白也毒性，LC_（50）为10.88μg/ mL。 S. Frugiperda Midgut的组织病理学分析在两种治疗中显示出3小时后上皮细胞定义的逐步丧失。在24h后处理，其中肠变化包括细胞质空泡化，上皮细胞的肥大，和在这两个杯状和柱状细胞的顶端区域囊泡形成。而且，刷界膜损坏，特别是在脚卵细胞中。

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