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首页> 外文期刊>Biological Control: Theory and Application in Pest Management >Selection and characterization of the Bacillus thuringiensis strains toxic to Spodoptera eridania (Cramer), Spodoptera cosmioides (Walker) and Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)
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Selection and characterization of the Bacillus thuringiensis strains toxic to Spodoptera eridania (Cramer), Spodoptera cosmioides (Walker) and Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

机译：苏云金芽孢杆菌对埃里螺旋藻（Cramer），夜蛾（Spodoptera cosmioides（Walker）和斜纹夜蛾（Spodoptera frugiperda）（Smith）（鳞翅目：夜蛾科）有毒

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Among the pests of cultivated plants, the Spodoptera species complex is one of the most important. This genus is composed of polyphagous insects that attack, among other crops, cotton, corn, soy and castor bean. In Brazil Spodoptera frugiperda has been recognized as a primary pest in cotton, and Spodoptera eridania (Cramer) and Spodoptera cosmioides (Walker) species are important pests in cotton and soy. This study was performed in 2008 and was focused on selecting and characterizing Bacillus thuringiensis strains highly pathogenic to S. cosmioides, S. eridania and S. frugiperda and identifying Cry proteins toxic to these species. One hundred strains that exhibited toxicity to Lepidoptera were evaluated through bioassay and the most toxic ones were characterized through morphologic, biochemical and molecular analyses and LC50 values were determined. Since the cry1Aa, cry1Ab, cry1Ac and cry2Aa genes were present in most of the toxic strains, recombinant Bt producing toxins encoded by representative genes from these families, were individually tested. The Cry proteins showed different levels of toxicity to the three Spodoptera species, with Cry1Aa and Cry1Ab the most toxic to S. cosmioides, Cry2Aa the most toxic to S. eridania and Cry1Aa, Cry1Ab and Cry2Aa most toxic to S. frugiperda. Cry1Ac, a component of some transgenic cotton varieties such as Bollgard I, extensively used in Brazil, presented low toxicity to the three species studied.
机译：在栽培植物的害虫中，斜纹夜蛾物种复合体是最重要的害虫之一。该属由攻击植物的多食性昆虫组成，其中包括棉花，玉米，大豆和蓖麻子。在巴西，斜纹夜蛾被认为是棉花中的主要害虫，而斜纹夜蛾（Cramer）和夜蛾（Spodoptera cosmioides）（沃克）是棉花和大豆中的重要害虫。这项研究于2008年进行，专注于选择和鉴定对cosmioides，S。eridania和S. frugiperda具有高致病性的苏云金芽孢杆菌菌株，并鉴定对这些物种有毒的Cry蛋白。通过生物测定评估了一百种对鳞翅目有毒的菌株，并通过形态，生化和分子分析表征了最具毒性的菌株，并确定了LC50值。由于cry1Aa，cry1Ab，cry1Ac和cry2Aa基因存在于大多数毒性菌株中，因此分别测试了由这些家族的代表性基因编码的重组Bt产生毒素。 Cry蛋白对三种斜纹夜蛾具有不同程度的毒性，其中Cry1Aa和Cry1Ab对毒死链球菌的毒性最大，Cry2Aa对毛鳞球菌和Cry1Aa的毒性最大，Cry1Ab和Cry2Aa对沙门氏菌的毒性最大。 Cry1Ac是一些转基因棉花品种的组成部分，例如在巴西广泛使用的Bollgard I，对这三个品种的毒性较低。

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