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首页> 外文期刊>Applied and Environmental Microbiology >Temporal Transcriptional Pattern of Three Melanin Biosynthesis Genes, PKS1, SCD1, and THR1, in Appressorium-Differentiating and Nondifferentiating Conidia of Colletotrichum lagenarium.
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Temporal Transcriptional Pattern of Three Melanin Biosynthesis Genes, PKS1, SCD1, and THR1, in Appressorium-Differentiating and Nondifferentiating Conidia of Colletotrichum lagenarium.

机译：炭疽菌的Appressorium-differentiated和non-differentiated分生孢子中的三个黑色素生物合成基因PKS1，SCD1和THR1的时间转录模式。

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摘要

A phytopathogenic fungus, Colletotrichum lagenarium, produces melanized appressoria that display temperature-sensitive differentiation. Conidia incubated in water at 24(deg)C germinated, and germ tubes differentiated into melanized appressoria. On the other hand, conidia incubated in water at 32(deg)C germinated and produced germ tubes that elongated without appressorium differentiation. Conidia in 0.1% yeast extract solution at 32(deg)C germinated and developed into vegetative hyphae. In this study, we investigated the temporal transcriptional pattern of cloned melanin biosynthesis genes, PKS1, SCD1, and THR1, in these differentiating and nondifferentiating conidia. During appressorium differentiation, de novo transcripts of the three melanin biosynthesis genes accumulated 1 to 2 h after the start of conidial incubation at 24(deg)C, and the amount of transcripts began to decrease at 6 h. In conidia germinating in water at 32(deg)C, the transcriptional pattern of the PKS1, SCD1, and THR1 genes was similar to that of these genes in appressorium-forming conidia, although no appressoria were formed. However, in conidia in 0.1% yeast extract solution at 32(deg)C, transcripts of the three melanin biosynthesis genes hardly accumulated.
机译：一种植物病原真菌，炭疽菌（Colletotrichum lagenarium），产生黑化的压感，表现出对温度敏感的分化。分生孢子在24℃的水中孵育后萌发，胚芽管分化为黑色化的压疮。另一方面，在32℃的水中孵育的分生孢子萌发并产生了胚芽管，其伸长而没有前庭分化。 0.1％酵母提取液中的分生孢子在32°C时萌发并发育为营养菌丝。在这项研究中，我们调查了在这些分化和非分化分生孢子中克隆的黑色素生物合成基因PKS1，SCD1和THR1的时间转录模式。在Appressorium分化过程中，在24℃分生孢子温育开始后1至2小时，三个黑色素生物合成基因的从头转录物积累，并且在6 h时转录物的数量开始减少。在32°C的水中萌发的分生孢子中，尽管没有形成贴壁动物，但PKS1，SCD1和THR1基因的转录模式与这些在贴壁动物的分生孢子中的基因相似。然而，在32℃下在0.1％酵母提取液中的分生孢子中，三个黑色素生物合成基因的转录物几乎不积累。

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