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首页> 外文期刊>Applied and Environmental Microbiology >Selection and Identification of a Listeria monocytogenes Target Strain for Pulsed Electric Field Process Optimization
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Selection and Identification of a Listeria monocytogenes Target Strain for Pulsed Electric Field Process Optimization

机译：用于脉冲电场过程优化的单核细胞增生李斯特菌靶标菌株的选择和鉴定

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Nine Listeria monocytogenes strains were treated individually with a continuous pulsed electric field (PEF) apparatus, and their sensitivities to the treatment were compared at 25 kV/cm. When cell suspensions of these strains in 0.1% NaCl (pH 7.0) were treated at 23°C for 144 μs, inactivation ranged from 0.7 to 3.7 log10 CFU/ml. Inactivation by 72-μs PEF treatments at 37°C ranged from 0.3 to 2.5 log10 CFU/ml. L. monocytogenes OSY-8578 was substantially more resistant than other strains when cells were PEF treated in 0.1% NaCl, whereas Scott A was one of the most sensitive strains. The superiority of OSY-8578's resistance to that of Scott A was confirmed in 50% diluted acid whey (pH 4.2). Changes in sensitivity to PEF during phases of growth were minimal in OSY-8578 and substantial in Scott A. Use of L. monocytogenes OSY-8578, therefore, is recommended in studies to optimize PEF processes that target L. monocytogenes. The nine L. monocytogenes strains were genotyped with pulsed-field gel electrophoresis (PFGE) and arbitrarily primed PCR (AP-PCR) techniques. These strains were better differentiated with PFGE than with AP-PCR. The target strain (OSY-8578) was characterized by both molecular typing techniques, but resistance to PEF, in general, was not associated with a particular genotype group.
机译：用连续脉冲电场（PEF）装置分别处理了9株单核细胞增生李斯特菌菌株，并在25 kV / cm的条件下比较了它们对治疗的敏感性。当这些菌株在0.1％NaCl（pH 7.0）中的细胞悬液在23°C下处理144μs时，失活范围为0.7至3.7 log 10 CFU / ml。在37°C下72μsPEF处理的失活范围为0.3至2.5 log 10 CFU / ml。 L。当用0.1％NaCl的PEF处理细胞时，单核细胞增生性OSY-8578的耐药性比其他菌株强得多，而Scott A是最敏感的菌株之一。在50％稀酸乳清（pH 4.2）中证实了OSY-8578的抗性优于ScottA。在生长阶段，对PEF敏感性的变化在OSY-8578中是最小的，在Scott A中是相当大的。使用 L。因此，在研究中建议使用单核细胞增生 OSY-8578来优化针对 L的PEF过程。单核细胞增生。九个 L。用脉冲场凝胶电泳（PFGE）和任意引物PCR（AP-PCR）技术对单核细胞增生李斯特菌菌株进行基因分型。 PFGE比AP-PCR更好地区分了这些菌株。两种分子分型技术都对目标菌株（OSY-8578）进行了表征，但对PEF的抗性通常与特定的基因型组无关。

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