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CO Dehydrogenase Genes Found in Metagenomic Fosmid Clones from the Deep Mediterranean Sea

机译：地中海深海超基因组化石克隆中发现的CO脱氢酶基因

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摘要

The use of carbon monoxide (CO) as a biological energy source is widespread in microbes. In recent years, the role of CO oxidation in superficial ocean waters has been shown to be an important energy supplement for heterotrophs (carboxydovores). The key enzyme CO dehydrogenase was found in both isolates and metagenomes from the ocean's photic zone, where CO is continuously generated by organic matter photolysis. We have also found genes that code for both forms I (low affinity) and II (high affinity) in fosmids from a metagenomic library generated from a 3,000-m depth in the Mediterranean Sea. Analysis of other metagenomic databases indicates that similar genes are also found in the mesopelagic and bathypelagic North Pacific and on the surfaces of this and other oceanic locations (in lower proportions and similarities). The frequency with which this gene was found indicates that this energy-generating metabolism would be at least as important in the bathypelagic habitat as it is in the photic zone. Although there are no data about CO concentrations or origins deep in the ocean, it could have a geothermal origin or be associated with anaerobic metabolism of organic matter. The identities of the microbes that carry out these processes were not established, but they seem to be representatives of either Bacteroidetes or Chloroflexi.
机译：一氧化碳（CO）作为生物能源的使用已在微生物中广泛使用。近年来，一氧化碳在浅层海水中的作用已被证明是异养生物（羧化植物）的重要能量补充。在海洋光合带的分离株和元基因组中都发现了关键的酶CO脱氢酶，在该区中，CO是通过有机物光解而连续产生的。我们还发现了一种基因密码，该基因编码来自地中海中3,000 m深度的宏基因组文库中的fosmid中的形式I（低亲和力）和II（高亲和力）。对其他宏基因组学数据库的分析表明，在中古生代和深海北太平洋以及该和其他海洋位置的表面上也发现了相似的基因（比例和相似性较低）。发现该基因的频率表明，这种能量产生的代谢在深海生境中至少与在光合作用区中一样重要。尽管没有关于海洋深处的CO浓度或起源的数据，但它可能具有地热起源或与有机物的厌氧代谢有关。尚未确定进行这些过程的微生物的身份，但它们似乎是拟杆菌或绿弯曲菌的代表。

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