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Comparative Study of Bacterial Groups within the Human Cecal and Fecal Microbiota

机译:人类盲肠和粪便微生物群中细菌群的比较研究

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The composition of the human cecal microbiota is poorly known because of sampling difficulties. Samples of cecal fluid from eight subjects were collected via an intestinal tube. Feces were also collected. Total anaerobes, facultative anaerobes, bifidobacteria, andBacteroides were enumerated by culture methods, and the predominant phylogenetic groups were quantified by molecular hybridization using a set of six rRNA-targeted probes. The numbers of strict anaerobes, bifidobacteria, Bacteroides, and members of the Clostridium coccoides group and Clostridium leptum subgroup were lower in the cecum. Facultative anaerobes represented 25% of total bacteria in the cecum versus 1% in the feces.
机译:由于采样困难,人类盲肠微生物群的组成知之甚少。通过肠管收集来自八名受试者的盲肠液样品。还收集了粪便。通过培养方法对总厌氧菌,兼性厌氧菌,双歧杆菌和拟杆菌进行计数,并使用一组六种靶向rRNA的探针通过分子杂交对主要的系统发生群进行定量。盲肠中严格厌氧菌,双歧杆菌,拟杆菌,以及Clostridium coccoides组和Leptum Leptum亚组的数量较少。兼性厌氧菌占盲肠细菌总数的25%,而粪便占1%。

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