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首页> 外文期刊>Applied and Environmental Microbiology >Microbial Community Succession during Lactate Amendment and Electron Acceptor Limitation Reveals a Predominance of Metal-Reducing Pelosinus spp.
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Microbial Community Succession during Lactate Amendment and Electron Acceptor Limitation Reveals a Predominance of Metal-Reducing Pelosinus spp.

机译：乳酸修正和电子受体限制期间的微生物群落演替揭示了减少金属的螺菌属的优势。

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The determination of the success of in situ bioremediation strategies is complex. By using controlled laboratory conditions, the influence of individual variables, such as U(VI), Cr(VI), and electron donors and acceptors on community structure, dynamics, and the metal-reducing potential can be studied. Triplicate anaerobic, continuous-flow reactors were inoculated with Cr(VI)-contaminated groundwater from the Hanford, WA, 100-H area, amended with lactate, and incubated for 95 days to obtain stable, enriched communities. The reactors were kept anaerobic with N2 gas (9 ml/min) flushing the headspace and were fed a defined medium amended with 30 mM lactate and 0.05 mM sulfate with a 48-h generation time. The resultant diversity decreased from 63 genera within 12 phyla to 11 bacterial genera (from 3 phyla) and 2 archaeal genera (from 1 phylum). Final communities were dominated by Pelosinus spp. and to a lesser degree, Acetobacterium spp., with low levels of other organisms, including methanogens. Four new strains of Pelosinus were isolated, with 3 strains being capable of Cr(VI) reduction while one also reduced U(VI). Under limited sulfate, it appeared that the sulfate reducers, including Desulfovibrio spp., were outcompeted. These results suggest that during times of electron acceptor limitation in situ, organisms such as Pelosinus spp. may outcompete the more-well-studied organisms while maintaining overall metal reduction rates and extents. Finally, lab-scale simulations can test new strategies on a smaller scale while facilitating community member isolation, so that a deeper understanding of community metabolism can be revealed.
机译：确定原位生物修复策略成功与否的过程很复杂。通过使用受控的实验室条件，可以研究各个变量（例如U（VI），Cr（VI）和电子供体和受体）对群落结构，动力学和金属还原电位的影响。用来自华盛顿州汉福德市100-H地区受Cr（VI）污染的地下水接种一式三份的厌氧连续流反应器，并用乳酸进行修饰，并孵育95天以获得稳定，丰富的群落。用N 2气（9 ml / min）冲洗顶部空间，使反应器保持厌氧状态，并在48小时的生成时间内喂入确定的培养基，其中添加了30 mM乳酸和0.05 mM硫酸盐。最终的多样性从12个门中的63个属减少到11个细菌属（从3个属）和2个古细菌属（从1个门）。最终社区由Pelosinus spp主导。醋杆菌属（Acetobacterium spp。）的含量较低，而其他生物含量较低，包括产甲烷菌。分离出四株新的Pelosinus菌株，其中三株能够还原Cr（VI），而一株也能还原U（VI）。在有限的硫酸盐条件下，看来硫酸盐还原剂（包括Desulfovibrio spp。）已被淘汰。这些结果表明在电子受体原位限制期间，诸如Pelosinus spp的生物体。在保持总体金属还原率和范围的同时，可能会胜过研究程度更高的生物。最后，实验室规模的模拟可以在促进社区成员隔离的同时，以较小的规模测试新策略，从而可以揭示对社区代谢的更深入了解。

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