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首页> 外文期刊>Applied and Environmental Microbiology >Production of l-Ascorbic Acid by Metabolically Engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii
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Production of l-Ascorbic Acid by Metabolically Engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii

机译：代谢工程酿酒酵母和百日酵母生产L-抗坏血酸

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摘要

Yeasts do not possess an endogenous biochemical pathway for the synthesis of vitamin C. However, incubated with l-galactose, l-galactono-1,4-lactone, or l-gulono-1,4-lactone intermediates from the plant or animal pathway leading to l-ascorbic acid, Saccharomyces cerevisiae and Zygosaccharomyces bailii cells accumulate the vitamin intracellularly. Overexpression of the S. cerevisiae enzymes d-arabinose dehydrogenase and d-arabinono-1,4-lactone oxidase enhances this ability significantly. In fact, the respective recombinant yeast strains even gain the capability to accumulate the vitamin in the culture medium. An even better result is obtainable by expression of the plant enzyme l-galactose dehydrogenase from Arabidopsis thaliana. Budding yeast cells overexpressing the endogenous d-arabinono-1,4-lactone oxidase as well as l-galactose dehydrogenase are capable of producing about 100 mg of l-ascorbic acid liter?1, converting 40% (wt/vol) of the starting compound l-galactose.
机译：酵母不具有合成维生素C的内源性生化途径。但是，应与来自植物或动物途径的l-半乳糖，l-半乳糖-1,4-内酯或l-gulono-1,4-内酯中间体一起孵育导致l-抗坏血酸，酿酒酵母和百日咳酵母细胞在细胞内积累维生素。酿酒酵母酶d-阿拉伯糖脱氢酶和d-阿拉伯糖基-1,4-内酯氧化酶的过表达显着增强了这种能力。实际上，各个重组酵母菌株甚至具有在培养基中积累维生素的能力。通过表达拟南芥的植物酶I-半乳糖脱氢酶可获得更好的结果。过表达内源性d-阿拉伯-1,4-内酯氧化酶以及l-半乳糖脱氢酶的芽生酵母细胞能够产生约100 mg的l-抗坏血酸升？1，转化了40％（重量/体积）的l-抗坏血酸。起始化合物1-半乳糖。

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