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首页> 外文期刊>Applied Microbiology >Comparison of the Elution and Absorption of Picornaviruses by Cotton and Calcium Alginate Wool Swabs
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Comparison of the Elution and Absorption of Picornaviruses by Cotton and Calcium Alginate Wool Swabs

机译：棉和藻酸钙棉签对小核糖核酸病毒的洗脱和吸收比较

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摘要

The recovery of picornaviruses by cotton and calcium alginate wool swabs was studied by use of prototype strains of poliovirus 1, echovirus 1, coxsackievirus A9, and rhinovirus 16/60. No significant differences in recovery of viruses by the two types of swabs could be demonstrated. It is suggested that use of cotton swabs in the virus laboratory be continued, since wool swabs may favor the recovery of undesirable bacterial contaminants. Adsorption of viruses by cotton and wool swabs was similar from virus-buffer mixtures at pH 4.0, 7.0, and 8.0 at 24 and 37 C. Elution of virus from cotton and wool swabs was also studied. There was no significant difference in the amount of virus eluted at pH 5.5, 7.1, or 8.4.
机译：通过使用脊髓灰质炎病毒1，回声病毒1，柯萨奇病毒A9和鼻病毒16/60的原型毒株研究了棉签和藻酸钙棉签对小核糖核酸病毒的回收。两种棉签在病毒回收方面均无明显差异。建议继续在病毒实验室中使用棉签，因为棉签可能有助于回收不良细菌污染物。在24和37℃下，pH 4.0、7.0和8.0的病毒-缓冲液混合物中棉签和棉签对病毒的吸附相似。还研究了棉签和棉签对病毒的洗脱。在pH 5.5、7.1或8.4下洗脱的病毒量没有显着差异。

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