Comparison of Different Denaturing Gradient Gel Electrophoresis Primer Sets for the Study of Marine Bacterioplankton Communities

Olga Sánchez; Josep M. Gasol; Ramon Massana; Jordi Mas; Carlos Pedrós-Alió

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Comparison of Different Denaturing Gradient Gel Electrophoresis Primer Sets for the Study of Marine Bacterioplankton Communities

机译：不同变性梯度凝胶电泳引物对海洋细菌性浮游生物群落研究的比较

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An annual seasonal cycle of composition of a bacterioplankton community in an oligotrophic coastal system was studied by denaturing gradient gel electrophoresis (DGGE) using five different primer sets. Analysis of DGGE fingerprints showed that primer set 357fGC-907rM grouped samples according to seasons. Additionally, we used the set of 16S rRNA genes archived in the RDPII database to check the percentage of perfect matches of each primer for the most abundant bacterial groups inhabiting coastal plankton communities. Overall, primer set 357fGC-907rM was the most suitable for the routine use of PCR-DGGE analyses in this environment.

机译：通过使用五个不同引物对梯度凝胶电泳（DGGE）进行变性，研究了贫营养型沿海系统中浮游细菌群落组成的年度季节性循环。 DGGE指纹图谱分析显示，引物组357fGC-907rM按季节分组。此外，我们使用RDPII数据库中存档的16S rRNA基因集来检查每种引物与沿海浮游生物群落中最丰富的细菌群体的完美匹配百分比。总体而言，引物组357fGC-907rM最适合在这种环境下常规使用PCR-DGGE分析。

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《Applied and Environmental Microbiology》 |2007年第18期|共6页
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Olga Sánchez; Josep M. Gasol; Ramon Massana; Jordi Mas; Carlos Pedrós-Alió;
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3. Bacterioplankton Community Structure in a Maritime Antarctic Oligotrophic Lake during a Period of Holomixis, as Determined by Denaturing Gradient Gel Electrophoresis (DGGE) and Fluorescence in Situ Hybridization (FISH) [J] . D. A. Pearce Microbial Ecology . 2003,第1期

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4. Microbial community analysis during composting of digested sludge and sawdust by using quinone profile and polymerase chain reaction-denaturing gradient gel electrophoresis [C] . Lijuan Yang, Mengchun Gao, Fangyuan Liang, The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议）论文集 . 2009

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5. Soil community analysis along a salt gradient using denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA genes. [D] . Dutta, Partha. 2006

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6. Comparison of Different Denaturing Gradient Gel Electrophoresis Primer Sets for the Study of Marine Bacterioplankton Communities [O] . Olga Sánchez, Josep M. Gasol, Ramon Massana, 2007

机译：不同变性梯度凝胶电泳引物对海洋细菌性浮游生物群落研究的比较
7. Comparison of Different Denaturing Gradient Gel Electrophoresis Primer Sets for the Study of Marine Bacterioplankton Communities [O] . Sánchez, Olga, Gasol, Josep M., Massana, Ramon 2007

机译：不同变性梯度凝胶电泳引物对海洋细菌性浮游生物群落研究的比较

Comparison of Different Denaturing Gradient Gel Electrophoresis Primer Sets for the Study of Marine Bacterioplankton Communities

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