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首页> 外文期刊>Applied and Environmental Microbiology >Rare Branched Fatty Acids Characterize the Lipid Composition of the Intra-Aerobic Methane Oxidizer “Candidatus Methylomirabilis oxyfera”
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Rare Branched Fatty Acids Characterize the Lipid Composition of the Intra-Aerobic Methane Oxidizer “Candidatus Methylomirabilis oxyfera”

机译：稀有支链脂肪酸表征需氧甲烷氧化器“ Candidatus Methylomirabilis oxyfera”的脂质组成

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The recently described bacterium “Candidatus Methylomirabilis oxyfera” couples the oxidation of the important greenhouse gas methane to the reduction of nitrite. The ecological significance of “Ca. Methylomirabilis oxyfera” is still underexplored, as our ability to identify the presence of this bacterium is thus far limited to DNA-based techniques. Here, we investigated the lipid composition of “Ca. Methylomirabilis oxyfera” to identify new, gene-independent biomarkers for the environmental detection of this bacterium. Multiple “Ca. Methylomirabilis oxyfera” enrichment cultures were investigated. In all cultures, the lipid profile was dominated up to 46% by the fatty acid (FA) 10-methylhexadecanoic acid (10MeC16:0). Furthermore, a unique FA was identified that has not been reported elsewhere: the monounsaturated 10-methylhexadecenoic acid with a double bond at the Δ7 position (10MeC16:1Δ7), which comprised up to 10% of the total FA profile. We propose that the typical branched fatty acids 10MeC16:0 and 10MeC16:1Δ7 are key and characteristic components of the lipid profile of “Ca. Methylomirabilis oxyfera.” The successful detection of these fatty acids in a peatland from which one of the enrichment cultures originated supports the potential of these unique lipids as biomarkers for the process of nitrite-dependent methane oxidation in the environment.
机译：最近描述的细菌“ Candidatus Methylomirabilis oxyfera”将重要的温室气体甲烷的氧化与亚硝酸盐的还原耦合在一起。钙的生态学意义由于我们鉴定该细菌存在的能力目前仅限于基于DNA的技术，因此仍未充分开发“羟甲基甲烷菌”。在这里，我们研究了“Ca。羟甲基甲烷菌”以鉴定新的，不依赖基因的生物标记物，以对该细菌进行环境检测。多个“ Ca.研究了羟甲基甲烷菌的富集培养。在所有培养物中，脂类谱主要由脂肪酸（FA）10-甲基十六烷酸（10MeC16：0）占主导。此外，鉴定出独特的FA，在其他地方未曾报道过：在Δ7位具有双键的单不饱和10-甲基十六碳烯酸（10MeC16：1Δ7），占总FA分布的10％。我们认为典型的支链脂肪酸10MeC16：0和10MeC16：1Δ7是“ Ca”脂质谱的关键和特征成分。羟甲基甲烷菌。”在泥炭地中成功地检测到这些脂肪酸，一种富集培养物起源于该泥炭地，支持这些独特的脂质作为生物标记物在环境中依赖亚硝酸盐的甲烷氧化过程中的潜力。

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