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首页> 外文期刊>Applied Microbiology >Formation of 6-Aminopenicillanic Acid, Penicillins, and Penicillin Acylase by Various Fungi
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Formation of 6-Aminopenicillanic Acid, Penicillins, and Penicillin Acylase by Various Fungi

机译：各种真菌形成6-氨基青霉酸，青霉素和青霉素酰基转移酶

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Several penicillin-producing fungi were examined for ability to produce 6-aminopenicillanic acid (6-APA) and penicillin acylase. 6-APA was found in corn steep liquor fermentations of Trichophyton mentagrophytes, Aspergillus ochraceous, and three strains of Penicillium sp. 6-APA was not detected in fermentations of Epidermophyton floccosum although penicillins were produced. 6-APA formed a large part of the total antibiotic production of T. mentagrophytes. The types of penicillins produced by various fungi were identified by paper chromatography, and it was found that all cultures produced benzylpenicillin. T. mentagrophytes and A. ochraceous showed increased yields of benzylpenicillin and the formation of phenoxymethylpenicillin in response to the addition to the fermentation medium of phenylacetic acid and phenoxyacetic acid, respectively. Washed mycelia of the three Penicillium spp. and two high penicillin-yielding strains of P. chrysogenum possessed penicillin acylase activity against phenoxymethylpenicillin. A. ochraceous, T. mentagrophytes, E. floccosum, and Cephalosporium sp. also had penicillin acylase activity against phenoxymethylpenicillin. Only two of the above fungi, T. mentagrophytes and E. floccosum, showed significant penicillin acylase activity against benzylpenicillin; in both cases it was very low. The acylase activity of A. ochraceous was considerably increased by culturing in the presence of phenoxyacetic acid. It is concluded that 6-APA frequently but not invariably accompanies the formation of penicillin, and that penicillin acylase activity against phenoxymethylpenicillin is present in all penicillin-producing fungi.
机译：检查了几种产生青霉素的真菌的产生6-氨基青霉酸（6-APA）和青霉素酰基转移酶的能力。在玉米毛癣菌，曲霉和三种青霉菌菌株的玉米浸液发酵中发现了6-APA。尽管产生了青霉素，但在絮状表皮发酵中未检测到6-APA。 6-APA构成了棉铃虫抗生素生产总量的很大一部分。通过纸色谱法鉴定了由各种真菌产生的青霉素的类型，发现所有培养物均产生苄青霉素。分别加入苯乙酸和苯氧乙酸的发酵培养基后，T。mentagrophytes和草A. ququeous分别显示出苄青霉素的收率提高和苯氧甲基青霉素的形成。洗净了三个青霉菌的菌丝体。两种产青霉素的高产青霉菌菌株均具有针对苯氧甲基青霉素的青霉素酰基转移酶活性。草，棉铃虫，絮状大肠杆菌和头孢属。还具有针对苯氧甲基青霉素的青霉素酰基转移酶活性。上述真菌中只有两种，即棉铃虫和絮状大肠杆菌，对青霉素有明显的青霉素酰基转移酶活性。在两种情况下，它都非常低。通过在苯氧乙酸的存在下培养，草曲霉的酰化酶活性大大提高。结论是6-APA经常但并非总是伴随着青霉素的形成，并且在所有产生青霉素的真菌中都存在针对苯氧甲基青霉素的青霉素酰基转移酶活性。

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