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Elevated-Temperature Technique for the Isolation of Salmonella from Streams

机译：高温分离沙门氏菌的技术

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摘要

With a modified Moore swab for sampling bacteria, Salmonella organisms were recovered consistently from surface waters when the enrichment broths of SBG-sulfa and tetrathionate and Brilliant Green Agar plates were incubated at 41.5 C. When an equal portion of the same swab sample was incubated at 37.0 C, no salmonellae were detected. By use of the elevated-temperature technique, salmonellae were recovered from stream sites having relatively low total coliform densities of 2,200 per 100 ml and fecal coliform densities of 220 per 100 ml. These pathogens were isolated in water sampled as far as 73 river miles and 4 days time of travel downstream from the source of pollution under an ice cover that averaged 2.5 ft (76.2 cm) in thickness.
机译：用改良的摩尔棉签采样细菌，当将SBG-磺胺和四硫酸盐的富集肉汤和Brilliant Green琼脂平板在41.5 C下孵育时，从地表水中可以稳定地回收沙门氏菌。在37.0℃下，未检测到沙门氏菌。通过使用高温技术，从具有相对较低的总大肠菌群密度为每100 ml 2200和粪便大肠菌群密度为每100 ml 220的流场中回收了沙门氏菌。这些病原体被隔离在采样水中，采样距离最长为73英里，污染源在平均厚度为2.5英尺（76.2厘米）的冰盖下向污染源下游移动了4天。

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