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Particle Size Distribution of Serratia marcescens Aerosols Created During Common Laboratory Procedures and Simulated Laboratory Accidents

机译:普通实验室程序和模拟实验室事故中产生的粘质沙雷氏菌气溶胶的粒径分布

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Andersen air samplers were used to determine the particle size distribution of Serratia marcescens aerosols created during several common laboratory procedures and simulated laboratory accidents. Over 1,600 viable particles per cubic foot of air sampled were aerosolized during blending operations. More than 98% of these particles were less than 5 μ in size. In contrast, 80% of the viable particles aerosolized by handling lyophilized cultures were larger than 5 μ. Harvesting infected eggs, sonic treatment, centrifugation, mixing cultures, and dropping infectious material produced aerosols composed primarily of particles in the 1.0- to 7.5-μ size range.
机译:安德森空气采样器用于确定几种常见实验室程序和模拟实验室事故中产生的粘质沙雷氏菌气溶胶的粒径分布。在混合操作过程中,每立方英尺采样的空气中有1,600多个可行颗粒被雾化。这些颗粒中有98%以上的颗粒尺寸小于5μ。相反,通过处理冻干的培养物而雾化的存活颗粒的80%大于5μ。收获受感染的卵,进行声波处理,离心,混合培养物以及滴下传染性物质,产生的气溶胶主要由尺寸在1.0-7.5μm范围内的颗粒组成。

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