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首页> 外文期刊>Applied and Environmental Microbiology >Size and UV Germicidal Irradiation Susceptibility of Serratia marcescens when Aerosolized from Different Suspending Media
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Size and UV Germicidal Irradiation Susceptibility of Serratia marcescens when Aerosolized from Different Suspending Media

机译：不同悬浮介质气溶胶化的粘质沙雷氏菌的大小和紫外线杀菌敏感性

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Experimental systems have been built in laboratories worldwide to investigate the influence of various environmental parameters on the efficacy of UV germicidal irradiation (UVGI) for deactivating airborne microorganisms. It is generally recognized that data from different laboratories might vary significantly due to differences in systems and experimental conditions. In this study we looked at the effect of the composition of the suspending medium on the size and UVGI susceptibility of Serratia marcescens in an experimental system built in our laboratory. S. marcescens was suspended in (i) distilled water, (ii) phosphate buffer, (iii) 10% fetal calf serum, (iv) phosphate-buffered saline (saline, 0.8% sodium chloride), and (v) synthetic saliva (phosphate-buffered saline with 10% fetal calf serum). At low humidity (36%), S. marcescens suspended in water-only medium was the most susceptible to UVGI, followed by those in serum-only medium. The count median diameters (CMDs) for culturable particles from water-only and serum-only media were 0.88 and 0.95 μm, respectively, with the measurements based on their aerodynamic behavior. The bacteria suspended in phosphate buffer, synthetic saliva, and phosphate-buffered saline had similar UVGI susceptibility and CMD at 1.0, 1.4, and 1.5 μm, respectively. At high humidity (68%) the CMD of the particles increased by 6 to 16%, and at the same time UVGI susceptibility decreased, with the magnitude of decrease related to the type of suspending medium. In conclusion, the choice of suspending medium influenced both size and UVGI susceptibility of S. marcescens. These data are valuable for making comparisons and deciding on the use of an appropriate medium for various applications.
机译：全球实验室已建立了实验系统，以研究各种环境参数对UV杀菌辐射（UVGI）灭活空气中微生物的功效的影响。通常认为，由于系统和实验条件的不同，来自不同实验室的数据可能会有很大的不同。在这项研究中，我们研究了悬浮介质的组成对粘质沙雷氏菌大小和UVGI敏感性的影响。将粘菌葡萄球菌悬浮于（i）蒸馏水，（ii）磷酸盐缓冲液，（iii）10％的胎牛血清，（iv）磷酸盐缓冲盐水（盐水，0.8％的氯化钠）和（v）合成的唾液（磷酸盐缓冲盐水，含10％的胎牛血清）。在低湿度（36％）下，悬浮于纯水培养基中的粘质沙雷氏菌对UVGI最敏感，其次是纯血清培养基。来自纯水和纯血清培养基的可培养颗粒的计数中值直径（CMD）分别为0.88和0.95μm，其测量基于其空气动力学行为。悬浮在磷酸盐缓冲液，合成唾液和磷酸盐缓冲盐水中的细菌在1.0、1.4和1.5μm处分别具有相似的UVGI敏感性和CMD。在高湿度（68％）下，颗粒的CMD增加6％至16％，同时UVGI敏感性降低，其下降幅度与悬浮介质的类型有关。总之，悬浮介质的选择会影响粘液链球菌的大小和UVGI敏感性。这些数据对于进行比较并决定针对各种应用程序使用适当的介质非常有用。

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