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首页> 外文期刊>Aerobiologia >Using selective media to assess aerosolization damage and ultraviolet germicidal irradiation susceptibility of Serratia marcescens
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Using selective media to assess aerosolization damage and ultraviolet germicidal irradiation susceptibility of Serratia marcescens

机译：使用选择性培养基评估粘质沙雷氏菌的雾化损害和紫外线杀菌敏感性

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摘要

This study determined whether selective media, McConkey agar (MC) and minimal salt glucose agar (MA) are suitable for monitoring aerosolization damage of airborne Serratia marcescens in our laboratory aerosol exposure system and assessed the relationship between bacterial culturability in these media and ultraviolet germicidal irradiation (UVGI) susceptibility of the bacteria. Two types of bacterial cultures were prepared. The first culture was taken from bacteria growing on Tryptic soy agar (TSA) as complete medium (fresh culture), which provided nearly 100% of MC/MA tolerant bacteria, while the second one was prepared from freezing the fresh culture (frozen culture), which produced 55 and 81% of MC and MA tolerant bacteria, respectively. We monitored bacterial culturability in TSA, MC and MA from these cultures in the nebulizer reservoir and bioaerosls collected on a six-stage Andersen cascade bio-impactor. The results indicated that both concentration and percentage of MC/MA tolerant bacteria maintained at a similar level during nebulization. For the bioaerosols, although the concentration recovered in the media from the fresh culture was higher than that from the frozen culture, the percentage of MC/MA tolerant bacteria was similar to that before aerosolization. We concluded that MC and MA are not suitable for monitoring aerosolization damage of the bacteria. Moreover, culturability of the bacteria in MC and MA has no effect on their survival after aerosolization. With respect to the bacterial susceptibility to UVGI, MC/MA sensitive and tolerant population as well as the fresh and frozen cultures showed the same susceptibility.
机译：这项研究确定了选择性培养基，麦康凯琼脂（MC）和最低盐葡萄糖琼脂（MA）是否适合监测我们实验室气溶胶暴露系统中气载粘质沙雷氏菌的气雾化损害，并评估了这些培养基中细菌可培养性与紫外线杀菌辐射之间的关系。 （UVGI）对细菌的敏感性。制备了两种类型的细菌培养物。第一种培养物是从胰蛋白酶大豆琼脂（TSA）上生长完全的培养基（新鲜培养物）中提取的细菌，可提供近100％的MC / MA耐性细菌，而第二种培养物是通过冷冻新鲜培养物（冷冻培养物）制备的，分别产生55％和81％的耐MC和MA细菌。我们从雾化器储罐中的这些培养物和在六级安徒生级联生物撞击器上收集的生物气雾中监测了TSA，MC和MA中的细菌可培养性。结果表明，在雾化期间，耐MC / MA的细菌的浓度和百分比都保持在相似的水平。对于生物气溶胶，尽管从新鲜培养物中回收的培养基浓度高于从冷冻培养物中回收的浓度，但耐MC / MA细菌的百分比与雾化之前相似。我们得出结论，MC和MA不适合监测细菌的气雾化损伤。此外，MC和MA中细菌的可培养性对其雾化后的存活率没有影响。关于细菌对UVGI的敏感性，对MC / MA敏感且能耐受的种群以及新鲜和冷冻的培养物都显示出相同的敏感性。

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