• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Energy and Environmental Protection >Culture media for enhanced chitinase production from Serratia marcescens strain JPP1 and stability of crude enzyme
【24h】

Culture media for enhanced chitinase production from Serratia marcescens strain JPP1 and stability of crude enzyme

机译：来自Serratia Marcescens菌株JPP1的培养基增强丁蛋白酶生产和粗酶的稳定性

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Serratia marcescens strain JPP1 was isolated from the peanut hulls in Jiangsu Province, China. The strain exhibited antagonistic activity against aflatoxins production and chitinolytic activity by producing chitinases. For chitinase production glucose was identified as best carbon source, peptone as organic nitrogen source while ammonium sulphate as the best inorganic nitrogen source led to the highest chitinase activity. Chitin served as an essential inducer and chitinase production reached the peak level after 1.2% of chitin concentration. The crude chitinase was highly thermostable and had activity in broad pH (2-11) and temperature (30-90°C) range. Ca and Mn ions enhanced the chitinase production, while Na and K were of inhibitory action not more than 5%, and Cu, Zn and Fe ions resulted in drastic enzyme inhibition ranged between 63.7 to 70.5%. Addition of EDTA, mercaptoethanol and Tween 80 had positive effect on chitinase production while SDS decreased the chitinase production by S. marcescens JPP1.
机译：Serratia marcescens菌株JPP1从中国江苏省的花生船体中分离出来。该菌株通过生产几章酶来表现出对黄曲霉毒素产生和依甲酸钠活性的拮抗活性。对于几丁质酶生产葡萄糖被鉴定为最佳碳源，蛋白胨作为有机氮源，而硫酸铵作为最佳无机氮源导致最高的几丁质酶活性。几丁酸作为必要的诱导剂，几丁质酶生产达到甲壳素浓度1.2％后达到峰值水平。粗丁酶高稳定，具有宽pH（2-11）和温度（30-90℃）范围的活性。 Ca和Mn离子增强了几丁质酶生产，而Na和k抑制作用不大于5％，Cu，Zn和Fe离子导致激烈的酶抑制在63.7至70.5％之间。添加EDTA，巯基乙醇和吐温80对几丁质酶生产具有阳性作用，而SDS通过S.Marcescens JPP1降低了几丁质酶生产。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号