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Production of Streptokinase in Continuous Culture

机译：连续培养中链激酶的产生

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摘要

A method for continuous cultivation of a β-hemolytic streptococcus, strain H 64, is described. The production of cells and streptokinase at various dilution rates, pH, and temperature were studied in a complex medium supplied with excess glucose. At pH 7.0, productivity of cells and streptokinase, as well as the yield constant with respect to glucose, all increased with increasing dilution rate in the range of 0.1 to 0.5 hr-1. The production of streptokinase was found to be a function of both growth rate and cell concentration. Although higher concentrations of streptokinase were obtained in experiments with batch cultures, the production of streptokinase in continuous cultures was found to be 2.3 times higher. The possible industrial application of a continuous production method is considered.
机译：描述了一种连续培养β-溶血性链球菌菌株H 64的方法。在提供有过量葡萄糖的复杂培养基中研究了各种稀释率，pH和温度下细胞和链激酶的产生。在pH 7.0时，细胞的生产率和链激酶以及相对于葡萄糖的产量常数都随着稀释率的增加而增加，范围为0.1到0.5 hr-1。发现链激酶的产生是生长速率和细胞浓度的函数。尽管在分批培养的实验中获得了更高浓度的链激酶，但连续培养的链激酶产量却提高了2.3倍。考虑了连续生产方法的可能的工业应用。

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