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首页> 外文期刊>Applied Microbiology >Comparison of Direct Plating Media for the Isolation and Enumeration of Enterococci in Certain Frozen Foods
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Comparison of Direct Plating Media for the Isolation and Enumeration of Enterococci in Certain Frozen Foods

机译：某些冷冻食品中肠球菌分离和计数的直接电镀培养基的比较

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A total of 15 agar media were examined for their yield, selectivity, readability, and simplicity of preparation and use. A thallium medium of Barnes was selected as the better of the high yield-fair selectivity type of medium and an azide-citrate medium of Reinbold appeared to be the better of the low yield-high selectivity type of medium. Sodium carbonate (optimal concentration, 0.20%) was found to increase recovery substantially when added to certain media, especially in the presence of 0.05% Tween 80. When these two ingredients were incorporated into a medium modified after Slanetz and Bartley, the resultant medium was superior to other media for the isolation and enumeration of enterococci in certain frozen foods, such as peas and hamburger, by the direct plating method.
机译：总共检查了15种琼脂培养基的产量，选择性，可读性以及制备和使用的简便性。选择Barnes的medium培养基作为高产量-中等选择性类型的培养基，而Reinbold的叠氮化柠檬酸盐培养基似乎是低产量-高选择性类型的培养基。发现将碳酸钠（最佳浓度，0.20％）添加到某些介质中时，尤其是在0.05％Tween 80存在下，大大提高了回收率。当将这两种成分掺入在Slanetz和Bartley之后改性的介质中时，所得的介质为通过直接电镀法，在某些冷冻食品（如豌豆和汉堡）中肠球菌的分离和计数优于其他培养基。

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