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首页> 外文期刊>Applied Microbiology >Influence of Platen Temperatures and Storage Conditions on the Survival of Freeze-dried Salmonella typhimurium
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Influence of Platen Temperatures and Storage Conditions on the Survival of Freeze-dried Salmonella typhimurium

机译：模板温度和保存条件对冻干鼠伤寒沙门氏菌存活的影响

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摘要

Salmonella typhimurium survived freeze-drying at a platen temperature of 120 F (48.9 C) and also, though to a much lesser degree, at 160 F (82.6 C). The extent of the survival at these temperatures was dependent on the composition of the model system employed. The incidence of damage immediately after freeze-drying was greater for cells dried at the higher platen temperature and was influenced by the composition of the menstruum in which the cells were dried. In model systems having protein-dominant isotherms, survival during subsequent storage depended greatly on relative humidity, with recovery highest at relative humidities below those corresponding to moisture contents at which a monomolecular layer is formed. In menstrua having a higher sugar content, survival was best at low relative humidities corresponding to a very low equilibrium moisture content in the model system used. Damage during storage tended to be a function of the composition of the gels in which the organisms were freeze-dried, and also depended greatly on the presence of air and on the relative humidity. The maximal percentage of damage usually occurred at the low relative humidities as storage time increased.
机译：鼠伤寒沙门氏菌在120 F（48.9 C）的平板温度下可以冻干，即使在160 F（82.6 C）的条件下也可以幸免。在这些温度下的存活程度取决于所采用的模型系统的组成。对于在较高压板温度下干燥的细胞，冷冻干燥后立即发生的损伤发生率更高，并且受干燥细胞的月经的组成的影响。在具有以蛋白质为主的等温线的模型系统中，后续存储过程中的存活率很大程度上取决于相对湿度，在相对湿度以下（对应于形成单分子层的水分含量）以下的相对湿度下，回收率最高。在糖含量较高的月经中，在相对湿度较低（对应的模型系统中平衡水分含量非常低）下，生存期最佳。储存过程中的损坏往往取决于其中微生物被冷冻干燥的凝胶的组成，并且还很大程度上取决于空气的存在和相对湿度。随着存储时间的增加，最大的损坏百分比通常发生在相对湿度较低的情况下。

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