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Microtiter Method for the Evaluation of Viable Cells in Bacterial Cultures

机译:微量滴定法评估细菌培养物中的活细胞

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A microtiter technique was investigated as a means of evaluating viable cells in bacterial cultures. Parallel experiments were performed employing the conventional agar plate method along with the microtiter procedure. Statistical analyses showed that the correlation between the two methods was highly significant. With this new method, many samples were analyzed simultaneously, and readable results were obtained in 12 to 15 hr. Other advantages of this method were substantial savings of time, space, and materials. Also, the applicability of this method to estimates of mixed bacterial populations was demonstrated by studying the associative growth of two bacterial cultures.
机译:研究了微量滴定技术,作为评估细菌培养物中活细胞的一种方法。使用常规琼脂平板法和微量滴定程序进行平行实验。统计分析表明,两种方法之间的相关性非常显着。使用这种新方法,可以同时分析许多样品,并在12到15小时内获得可读的结果。该方法的其他优点是可以节省大量的时间,空间和材料。同样,通过研究两种细菌培养物的联合生长,证明了该方法对混合细菌种群估计的适用性。

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