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首页> 外文期刊>Applied Microbiology >Comparison by Gas-Liquid Chromatography of the Fatty Acids of Mycobacterium avium and Some Other Nonphotochromogenic Mycobacteria
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Comparison by Gas-Liquid Chromatography of the Fatty Acids of Mycobacterium avium and Some Other Nonphotochromogenic Mycobacteria

机译：气相色谱法比较禽分枝杆菌和其他一些非光致变色分枝杆菌的脂肪酸

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Fifty-eight strains of nonphotochromogenic mycobacteria representing nine different serotypes were studied, including 38 strains of Mycobacterium avium and 20 strains of Battey bacilli. The lipids were extracted from whole cells, saponified with potassium hydroxide, and esterified with diazomethane. The fatty-acid profiles determined by gas-liquid chromatography included saturated fatty acids ranging from C8 to C24, plus some unsaturated analogues and a branched-chain acid. No consistent differences in the fatty-acid profiles were observed between strains of M. avium and Battey bacilli. Quantitative differences in the means (significant at P < 0.01) were observed in the relative amounts of 14:0, 16:1, 18:0, 18:1, and a branched-chain acid among strains of the same serotype. We were unable to differentiate the fatty-acid profiles of recently isolated strains or those maintained in culture for more than 2 years.
机译：研究了代表9种不同血清型的58种非光致变色分枝杆菌，其中包括38株鸟分枝杆菌和20株百特杆菌。从全细胞中提取脂质，用氢氧化钾皂化，并用重氮甲烷酯化。通过气-液色谱法测定的脂肪酸谱包括C8至C24的饱和脂肪酸，以及一些不饱和类似物和支链酸。在鸟分枝杆菌和百特杆菌的菌株之间没有观察到一致的脂肪酸谱差异。在相同血清型的菌株中，相对量的14：0、16：1、18：0、18：1和支链酸的均值存在定量差异（在P <0.01时显着）。我们无法区分最近分离出的菌株或培养超过2年的菌株的脂肪酸谱。

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