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Nonlethal Freezing Injury to Metabolism and Motility of Pseudomonas fluorescens and Escherichia coli

机译:非致死性冷冻对荧光假单胞菌和大肠杆菌的代谢和运动性的伤害

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Some specific effects of the conditions of freezing on death and metabolic injury of a psychrophilic and a mesophilic species of bacteria were investigated, with particular respect to changes in motility. It was found that the extent of nonlethal metabolic freezing injury and the changes in motility are apparently correlated and are affected by the time and temperature of storage and the nature and pH of the suspending fluid. There were significant differences between the resistance to cold of the test organisms, the mesophilic being the more sensitive. The motility of the psychrophilic Pseudomonas fluorescens decreased considerably less after low-temperature exposure than did that of the mesophilic Escherichia coli. The percentage of restoration in motility of the subcultures was lower after freezing in water than in other suspending fluids.
机译:研究了冷冻条件对嗜冷和嗜温细菌的死亡和代谢损伤的一些特定影响,特别是关于运动性的变化。发现非致命性代谢性冷冻损伤的程度和运动性的变化显然是相关的,并且受储存时间和温度以及悬浮液的性质和pH的影响。测试微生物对寒冷的抵抗力之间存在显着差异,其中嗜温菌则更为敏感。低温暴露后,嗜冷荧光假单胞菌的运动性比中温大肠杆菌降低了。在水中冷冻后,亚培养物活力恢复的百分比低于其他悬浮液。

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