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Comparison of Several Methods for Preserving Bacteriophages

机译：几种噬菌体保存方法的比较

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A wide variety of bacteriophages were processed and stored under different conditions to compare methods for long-term preservation. Specimens were stored for 2 years at room temperature (24 to 28 C) and at 4 C as broth lysates in 50% glycerol, dried, and freeze-dried. Titers determined after processing indicated that, of the broth, glycerol, and freeze-dry methods, freeze-drying was most damaging to the phages tested, glycerol less damaging, and the broth method least damaging. After 2 years, titers of broth lysates were generally higher than those of glycerol or freeze-dried preparations. Dried preparations generally did not prove satisfactory. Preparations stored at 4 C showed better titers than those kept at room temperature. All titers declined with time regardless of the conditions of preservation.
机译：各种噬菌体均在不同条件下加工和储存，以比较长期保存的方法。将样品在50％甘油中的肉汤裂解液中于室温（24至28 C）和4 C下保存2年，干燥并冷冻干燥。加工后测定的滴度表明，在肉汤，甘油和冻干方法中，冻干对测试的噬菌体的损害最大，甘油的损害较小，而肉汤方法的损害最小。 2年后，肉汤裂解物的滴度通常高于甘油或冻干制剂的滴度。干燥的制剂通常不能令人满意。储存在4 C下的制剂显示出比室温下更好的滴度。无论保存条件如何，所有滴度均随时间下降。

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